Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein
Joris, Bernard; Ledent, P.; Kobayashi, T.et al.
1990 • In FEMS Microbiology Letters, 58 (1), p. 107-113
[en] A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 amino acid BLAR sensory-transducer involved in beta-lactamase inducibility in Bacillus licheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E. coli in the form of a water-soluble, Mr 26,000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Ledent, P.; Université de Liège - ULiège > Centre d'ingénierie des protéines
Kobayashi, T.; Waksman Institute of Microbiology
Lampen, J.-O.; Waksman Institute of Microbiology
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein
Publication date :
15 June 1990
Journal title :
FEMS Microbiology Letters
ISSN :
0378-1097
eISSN :
1574-6968
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
58
Issue :
1
Pages :
107-113
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE] Fond de la Recherche de la Faculté de Médecine ULg National Institutes for Allergy and Infectious Deseases
MacNab Escherichia coli and Salmonella typhimurium cellular and molecular biology , F.C. Neidhardt, J.L. Ingraham, K. Brooks Low, B. Magasanik, M. Schaechter, H.E. Umbarger, American Society for Microbiology, Washington, D.C; 1987, 1:732-759.
Bollinger, Park, Harayama, Hazelbauer Proc. Natl. Acad. Sci. U.S.A. 1984, 81:3287-3291.