Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein

Joris, Bernard; Ledent, P.; Kobayashi, T.; Lampen, J.-O.; Ghuysen, Jean-Marie

doi:10.1016/0378-1097(90)90111-3

Article (Scientific journals)

Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein

Joris, Bernard; Ledent, P.; Kobayashi, T. et al.

1990 • In FEMS Microbiology Letters, 58 (1), p. 107-113

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/91657

DOI
10.1016/0378-1097(90)90111-3

PubMed
2204571

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Keywords :

beta-lactamase induction; Escherichia coli; Bacillus Licheniformis; BLAR-CTD

Abstract :

[en] A cloning vector has been constructed which allows production and export by Escherichia coli of the Met346-Arg601 carboxy terminal domain of the 601 amino acid BLAR sensory-transducer involved in beta-lactamase inducibility in Bacillus licheniformis. The polypeptide, referred to as BLAR-CTD, accumulates in the periplasm of E. coli in the form of a water-soluble, Mr 26,000 penicillin-binding protein. These data and homology searches suggest that BLAR has a membrane topology similar to that of other sensory-transducers involved in chemotaxis.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Ledent, P.; Université de Liège - ULiège > Centre d'ingénierie des protéines

Kobayashi, T.; Waksman Institute of Microbiology

Lampen, J.-O.; Waksman Institute of Microbiology

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Expression in Escherichia Coli of the Carboxy Terminal Domain of the Blar Sensory-Transducer Protein of Bacillus Licheniformis as a Water-Soluble Mr 26,000 Penicillin-Binding Protein

Publication date :

15 June 1990

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Issue :

Pages :

107-113

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRSM - Fonds de la Recherche Scientifique Médicale
Fond de la Recherche de la Faculté de Médecine ULg
National Institutes for Allergy and Infectious Deseases

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since 25 May 2011

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