[en] The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lepage, Sophie; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Dubois, Philippe ; Université de Liège - ULiège > Institut de Chimie > Centre d'Ingénierie des Protéines
Ghosh, Thushar-Kanti; Université de Liège - ULiège > Institut de Cimie > Centre d'Ingénierie des Protéines
Joris, Bernard ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Mahapatra, Sebabrata; Bose Institute-Calcutta > Department of Chemistry
Kundu, Manikuntula; Bose Institute-Calcutta > Department of Chemistry
Basu, Joyoti; Bose Institute-Calcutta > Department of Chemistry
Chakrabarti, Parul; Bose Institute-Calcutta > Department of Chemistry
Cole, Stewart-T; Institut Pasteur (Paris) > Unite de Génétique Moléculaire Bactérienne
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