[en] Studying the muscular hypertrophy of Texel sheep by forward genetics, we have identified an A-to-G transition in the 3'UTRof the GDF8 gene that reveals an illegitimate target site for microRNAs miR-1 and miR-206 that are highly expressed in skeletal muscle. This causes the down-regulation of this muscle-specific chalone and hence contributes to the muscular hypertrophyof Texel sheep. We demonstrate that polymorphisms which alter the content of putative miRNA target sites are commonin human and mice, and provide evidence that both conserved and nonconserved target sites are selectively constrained. Wespeculate that these polymorphisms might be important mediators of phenotypic variation including disease. To facilitatestudies along those lines, we have constructed a database (www.patrocles.org) listing putative polymorphic microRNA–targetinteractions.
Research Center/Unit :
Systmod
Disciplines :
Computer science Genetics & genetic processes
Author, co-author :
Georges, Michel ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Clop, Alex; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Marcq, Fabienne ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Takeda, Haruko ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Pirottin, Dimitri ; Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Tordoir, Xavier ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Hiard, Samuel ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Bibé, B.; Institut National de la Recherche Agronomique > Station d'Amélioration Génétique des Animaux
Bouix, J.; Institut National de la Recherche Agronomique > Station d'Amélioration Génétique des Animaux
Caiment, Florian ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Eychenne, F.; Institut National de la Recherche Agronomique > Station d'Amélioration Génétique des Animaux
Larzul, C.; Institut National de la Recherche Agronomique > Station d'Amélioration Génétique des Animaux
Laville, E.; Institut National de la Recherche Agronomique > Station d'Amélioration Génétique des Animaux
Meish, F.; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
Milenkovic, D.; Institut Scientifique de Recherche Agronomique - INRA > Faculté des sciences
Tobin, J.; University of Cambridge > Wyeth Research > Cardiovascular and Metabolic Diseases
Charlier, Carole ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale
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