Article (Scientific journals)
The peptidoglycan in walls of Butyribacterium rettgeri
Guinand, Micheline; Ghuysen, Jean-Marie; Schleifer, Karl H. et al.
1969In Biochemistry, 8 (1), p. 200-207
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Keywords :
alanine; amidohydrolases; amino acid sequence; amino acids; cell wall; chromatography; eubacterium; glutamates; hexosamines; lysine; muramidase; ornithine; peptide Biosynthesis; peptide hydrolases; polysaccharides; proteins; serine; streptomyces; analysis; gel; thin layer; bacterial
Abstract :
[en] The wall peptidoglycan in Butyribacterium rettgeri is composed of Nα-(L-seryl-γ-D-glutamyl)-L-ornithyl-D-alanine subunits. The peptide subunits are cross-linked by means of a D-lysine or a D-ornithine residue, extending from the α-carboxyl group of the glutamic acid of one peptide subunit, to which D-lysine is linked through its є-amino group or D-ornithine through its δ-amino group, to the carboxyl group of the C-terminal D-alanine residue of another peptide subunit. D-Lysine and D-ornithine bridges occur in the ratio of 2:1. The Streptomyces KM endopeptidase hydrolyses the Nα-( D-alanyl)-D-lysine and Nα-(D-alanyl)-D-ornithine linkages and causes wall solubilization. The walls, as they are prepared, have a low degree of cross-linking. About 59% of the peptide subunits occur as monomers (18%), dimers (24%), and trimers (17%). Higher oligomers account for the remaining 41 %. The disaccharide units obtained after sequential treatment with Streptomyces F1 endo-N-acetylmuramidase and Streptomyces N-acetylmuramyl-L-alanine amidase are all β-1,4-N-acetylglucosaminyl-N-acetylmuramic acid.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Guinand, Micheline;  Faculté de Sciences de Lyon > Service de Chimie Biologique
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Service de Bactériologie
Schleifer, Karl H.;  Technische Hochschule - München > Institut für Angewandte Botanik
Kandler, Otto;  Technische Hochschule - München > Institut für Angewandte Botanik
Language :
English
Title :
The peptidoglycan in walls of Butyribacterium rettgeri
Publication date :
01 January 1969
Journal title :
Biochemistry
ISSN :
0006-2960
eISSN :
1520-4995
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Volume :
8
Issue :
1
Pages :
200-207
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
Available on ORBi :
since 17 February 2011

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