[en] The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Malissard, Martine; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Guinand, Micheline; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Vacheron, Marie-Jeanne; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Michel, Georges; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Marty, Nicole; Université Paul Sabatier - Toulouse 3 - UPS > Laboratoire de Microbiologie
Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines
Thamm, Iris ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate
Publication date :
1993
Journal title :
FEMS Microbiology Letters
ISSN :
0378-1097
eISSN :
1574-6968
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
110
Issue :
1
Pages :
101-106
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale F.R.S.-FNRS - Fonds de la Recherche Scientifique
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