[en] The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Malissard, Martine; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Guinand, Micheline; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Vacheron, Marie-Jeanne; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Michel, Georges; Université Claude Bernard - Villeurbanne (France) > Laboratoire de Biochimie Microbienne
Marty, Nicole; Université Paul Sabatier - Toulouse 3 - UPS > Laboratoire de Microbiologie
Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines
Thamm, Iris ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate
Publication date :
1993
Journal title :
FEMS Microbiology Letters
ISSN :
0378-1097
eISSN :
1574-6968
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
110
Issue :
1
Pages :
101-106
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Skjåk-Braek, Alginates: biosyntheses and some structure-function relationships relevant to biomedical and biotechnological applications. (1992) Biochem Soc Trans, 20, pp. 27-33
Gacesa, (1992) Int. J. Biochem., 24, pp. 545-552
Skjåk-Braek, Grasdalen, Larsen, (1986) Carbohydr. Res., 154, pp. 239-250
Ramphal, Pier, (1985) Infect. Immun., 47, pp. 1-4
Simpson, Smith, Dean, (1988) J. Gen. Microbiol., 134, pp. 29-36
Nichols, Evans, Slack, Walmsley, (1989) J. Gen. Microbiol., 135, pp. 1291-1303
Gacesa, Russel, (1990) Pseudomonas Infection and Alginates: Biochemistry, Genetics and Pathology, p. 233. , Chapman and Hall, London
Pederson, Are Intestinal bacteria involved in the etiology of rheumatoid arthritis? (1992) APMIS, 100, pp. 1-79
Bayer, Speert, Park, Tu, Witt, Nast, Norman, (1991) Infect. Immun., 59, pp. 302-308
Bayer, Park, Ramos, Nast, Eftekhar, Schiller, (1992) Infect. Immun., 60, pp. 3979-3985
Mai, Seow, Pier, McCormack, Thong, (1993) Infect. Immun., 61, pp. 559-564
Brown, Preston, Ingram, (1991) Appl. Environ. Microbiol., 57, pp. 1870-1872
Caswell, Gaseca, Lutrell, Weightman, (1989) Gene, 75, pp. 127-134
Yonemoto, Yamaguchi, Kimura, Sakaguchi, Okayama, Murata, (1992) J. Ferment. Bioeng., 73, pp. 225-227
Gaseca, Goldberg, (1992) J. Gen. Microbiol., 138, pp. 1665-1670
Romeo, Preston, (1986) Biochemistry, 25, pp. 8385-8391
Von Riesen, (1980) Appl. Environ. Microbiol., 39, pp. 92-96
Karkhanis, Zeltner, Jackson, Carlo, (1978) Anal. Biochem., 85, pp. 595-601
Bradford, (1976) Anal. Biochem., 72, pp. 248-254
Laemmli, Favre, (1973) J. Mol. Biol., 80, pp. 575-599
Matsudaira, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. (1987) J Biol Chem, 262, pp. 10035-10038
Maniatis, Fritsch, Sambrook, (1982) Molecular Cloning: A Laboraoory Manual, , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Sanger, Nicklen, Coulson, (1977) Proc. Natl. Acad. Sci. USA, 74, pp. 5463-5467