Reference : Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomy...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models
Frère, Jean-Marie mailto [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Perkins, Harold R [University of Liverpool > Department of Microbiology > > >]
European Journal of Biochemistry
Blackwell Science
Yes (verified by ORBi)
United Kingdom
[en] acyltransferases/*metabolism ; bnding sites ; binding, competitive ; carbenicillin/metabolism ; carboxypeptidases/*metabolism ; kinetics ; mathematics ; models, chemical ; penicillin g/metabolism ; penicillin v/metabolism ; penicillins/*metabolism ; peptidyl transferases/*metabolism ; protein binding ; streptomyces/*enzymology
[en] The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver-Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver-Burk plots can be obtained on the basis of a non-competitive type of inhibition. At present, a choice between the two models cannot be made.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fond de la Recherche Fondamentale Collective
Researchers ; Professionals

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