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28 January 2011
Article (Scientific journals)
Kinetics of interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics. A choice of models
Frère, Jean-Marie  ; Ghuysen, Jean-Marie  ; Iwatsubo, Motohiro
1975 • In European Journal of Biochemistry, 57 (2), p. 343-351
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Keywords :
acyltransferases/*metabolism; ampicillin/metabolism; binding sites; carbenicillin/metabolism; carboxypeptidases/*metabolism; kinetics; mathematics; penicillin g/metabolism; penicillins/*metabolism; peptidyl transferases/*metabolism; protein binding; streptomyces/*enzymology
Abstract :
[en] The simplest model for the interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics involves the three following steps: (a) the formation of a reversible equimolar enzyme - antibiotic complex; (b) the irreversible transformation of this complex into a modified enzyme - antibiotic complex; and (c) the breakdown of this latter complex and the concomitant release of a regenerated enzyme and a modified antibiotic molecule. The dissociation constant for step 1 and the rate constants for steps 2 and 3 were measured with various beta-lactam antibiotics. With antibiotic such as benzylpenicillin, which behaves as a good 'substrate', steps 1 and 2 occur at enzymic velocities, whereas step 3 occurs at a very low velocity and hence is responsible for the low efficiency of the overall process.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Frère, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie
Iwatsubo, Motohiro;  Centre National de la Recherche Scientifique - CNRS > Centre de Génetique Moléculaire
Language :
English
Title :
Kinetics of interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics. A choice of models
Publication date :
15 September 1975
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
57
Issue :
2
Pages :
343-351
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
FRFC - Fonds de la Recherche Fondamentale Collective

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