Reference : Enzymatic method for rapid and sensitive determination of β-lactam antibiotics
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/83036
Enzymatic method for rapid and sensitive determination of β-lactam antibiotics
English
Frère, Jean-Marie mailto [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > >]
Klein, Daniel [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Ghuysen, Jean-Marie [Université de Liège - ULiège > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
1-Oct-1980
Antimicrobial Agents and Chemotherapy
American Society for Microbiology (ASM)
18
4
506-510
Yes (verified by ORBi)
International
0066-4804
1098-6596
Washington
DC
[en] animals ; anti-bacterial agents/*analysis/blood ; carboxypeptidases/antagonists & inhibitors/*diagnostic use ; cattle ; female ; humans ; kinetics ; milk/analysis ; penicillin g/analysis
[en] A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk.
Fonds de la Recherche Scientifique Médicale - FRSM
Researchers ; Professionals
http://hdl.handle.net/2268/83036

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