The importance of the negative charge of β-lactam compounds for the inactivation of the active-site serine DD-peptidase of Streptomyces R61

[en] The interaction between the Streptomyces R61 penicillin-sensitive DD-peptidase and deacetyl-cephalosporin C or its lactone derivative has been studied at different pH values. The results show the importance of an enzyme group of pK approximately equal to 9 which might form an ion pair with the free carboxylate of the former compound. This electrostatic interaction is shown to contribute to the formation of the first, non-covalent enzyme-inactivator complex by a factor of at least 50.

Disciplines :

Microbiology
Biochemistry, biophysics & molecular biology

Author, co-author :

Varetto, Louis ; Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie

Frère, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie

Language :

English

Title :

The importance of the negative charge of β-lactam compounds for the inactivation of the active-site serine DD-peptidase of Streptomyces R61

Publication date :

10 December 1987

Journal title :

FEBS Letters

ISSN :

0014-5793

eISSN :

1873-3468

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

225

Issue :

1-2

Pages :

218-222

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRSM - Fonds de la Recherche Scientifique Médicale

Available on ORBi :

since 07 January 2011

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Bibliography

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Frère, Leyh-Bouille, Ghuysen, Nieto, Perkins Methods Enzymol. 1976, 45 B:610-636.
Frère, Ghuysen, Iwatsubo Eur. J. Biochem. 1975, 57:343-351.
Frère, Kelly, Klein, Ghuysen, Claes, Vanderhaeghe Biochem. J. 1982, 203:223-234.
Faraci, Pratt Biochem. J. 1986, 238:309-312.

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