The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region.

Peptidase; Bacteria; Actinomycetes; Actinomycetales; Streptomycetaceae; Enzyme; Streptomyces; Column chromatography; Radiolabelling; N terminal-Sequence; Active site; Penicillin; Binding protein; Protéine de liaison; Bactérie; Chromatographie colonne; Marquage radioisotopique; Séquence N terminale; Site actif; Pénicilline; Proteina de enlace; Enzima; Cromatografía columna; Marcación radioisotópica; Secuencia N terminal; Lugar activo

Abstract :

[en] The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high homology with that of other penicillin-interactive proteins or domains. The active-site serine residue of the conserved tetrad Ser-Xaa-Xaa-Lys occurs at position 35. There is no indication for the presence of a signal peptide or an N-terminal hydrophobic sequence, suggesting that the Streptomyces K15 enzyme is probably anchored to the membrane by a C-terminal peptide segment.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Leyh-Bouille, Mélina; Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie

Vanbeeumen, Jozef; Rijksuniversiteit-Gent > Laboratorium voor Microbiologie

Renier-Pirlot, Suzanne; Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie

Joris, Bernard ; Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie

Nguyen-Distèche, Martine ; Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie

Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie

Language :

English

Title :

The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region.

Publication date :

1989

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, London, United Kingdom

Volume :

260

Issue :

Pages :

601-604

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRSM - Fonds de la Recherche Scientifique Médicale
Fond de la Recherche de la Faculté de Médecine ULg

Available on ORBi :

since 07 January 2011

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