[en] Study of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution yielded the following molecular parameters: radius of gyration R = 1.82 +/- 0.05 nm; largest diameter D = 5.9 +/- 0.2 nm; relative molecular mass Mr = 17000 +/- 2000; volume V approximately equal to 35 +/- 2 nm3; degree of hydration: 0.25 +/- 0.02 g water/g protein. By reference to theoretical scattering curves of rigid triaxial homogeneous bodies, a model which fits all experimental data is an elliptical cylinder. Such a model is compatible with that observed in the crystal structure. At those high concentrations necessary to form inactive enzyme-ligand associations the non-competitive beta-lactam inhibitors, cephalothin and cephalosporin C, drastically altered the scattering behaviour of the protein.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Labischinski, Harald; Robert-Koch-Institut des Bundesgesundheitsamtes
Giesbrecht, Peter; Robert-Koch-Institut des Bundesgesundheitsamtes
Fischer, E.; Institut Für Kristallographie der Freien Universität Berlin
Barnickel, G.; Institut Für Kristallographie der Freien Universität Berlin
Bradaczek, H.; Institut Für Kristallographie der Freien Universität Berlin
Frère, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Houssier, Claude ; Université de Liège - ULiège > Institut de Chimie > Laboratoire de Chimie Physique
Charlier, Paulette ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Dideberg, Otto; Université de Liège - ULiège > Institut de Physique > Laboratoire de Cristallographie
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Language :
English
Title :
Study of the Zn-containing DD-carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution.
Publication date :
1984
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
138
Issue :
1
Pages :
83-87
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Ghuysen J.M., Frère J.‐M., Leyh‐Bouille M., Dideberg O., Lamotte‐Brasseur J., Perkins H.R., De Coen J.L. Topics in Molecular Pharmacology , Burgen, A. S. V., Roberts, G. C. K., eds, pp., Elsevier/North‐Holland Biomedical Press; 1981, 63-97.
Labischinski H., Barnickel G., Bradaczek H., Giesbrecht P. Eur. J. Biochem. 1979, 95:147-155.
Barnickel G., Labischinski H., Bradaczek H., Giesbrecht P. Eur. J. Biochem. 1979, 95:157-165.
Kelly J.A. (1983) The Murein Sacculus of Bacterial Cell Walls. Architecture and Growth. International FEMS Symposium. March 13–18, 1983 , Hakenbeck, R., Höltje, J. V., Labischinski, H., eds, pp., Walter de Gruyter and Co, Berlin, New York; 387-392.
Joris B., Van Beeumen J., Casagrande F., Gerday Ch., Frère J.‐M., Ghuysen J.M. Eur. J. Biochem. 1983, 130:53-69.
Dideberg O., Charlier P., Dupont L., Vermeire M., Frère J.‐M., Ghuysen J.M. FEBS Lett. 1980, 117:212-214.
Charlier P., Dideberg O., Dive G., Dusart J., Frère J.‐M., Ghuysen J.M., Joris B., Lamotte‐Brasseur J., Leyh‐Bouille M., Nguyen‐Distèche M. The Murein Sacculus of Bacterial Cell Walls. Architecture and Growth. International FEMS Symposium. March 13–18, 1983 , Hakenbeck, R., Höltje, J. V., Labischinski, H., eds, pp., Walter de Gruyter and Co, Berlin, New York; 1983, 369-386.
Duez C., Frère J.‐M., Klein D., Noël M., Ghuysen J.M., Delcambe L., Dierickx L. Biochem. J. 1981, 193:75-82.
Glatter O. (1974) A new iterative method for collimation correction in small-angle scattering. Journal of Applied Crystallography 7:147-153.
Glatter O. (1977) A new method for the evaluation of small-angle scattering data. Journal of Applied Crystallography 10:415-421.
Labischinski H., Bradaczek H. (1977) A computer program using a fast Fourier transform algorithm for the calculation of X-ray scattering data from a variety of models. Journal of Applied Crystallography 10:363-364.
Kratky O., Leopold H., Stabinger H. Angew. Phys. 1969, 27:273-277.
Fredericq E., Houssier C. Biopolymers 1972, 11:2281-2308.
Wahl P., Weber G. J. Mol. Biol. 1967, 30:371-384.
Guinier C.R. C. R. Hebd. Séances Acad. Sci. 1937, 204:1115.
Schackman K.H. Methods Enzymol. 1957, 4:32-103.
Kratky O., Porod G., Kahovec I. Elektrochemie 1959, 55:53-59.
Porod G. Kolloid Z. 1952, 125:51-122.
Glatter O. (1979) The interpretation of real-space information from small-angle scattering experiments. Journal of Applied Crystallography 12:166-175.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.