Crystallization and X-ray diffraction study of the Streptomyces K15 penicillin-binding DD-transpeptidase.

Bacterial Proteins; Carrier Proteins/chemistry/isolation & purification/metabolism; Crystallization; Crystallography, X-Ray; Hexosyltransferases; Mass Spectrometry; Muramoylpentapeptide Carboxypeptidase/chemistry/isolation & purification/metabolism; Penicillin-Binding Proteins; Penicillins/metabolism; Peptidyl Transferases; Streptomyces/enzymology

Abstract :

[en] The 262 amino acid residue long DD-transpeptidase/penicillin-binding protein of Streptomyces K15 has been crystallized at room temperature by using the hanging drop vapour diffusion technique. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell parameters a = 46.4 A, b = 54.1 A and c = 108.3 A. They contain one protein molecule per asymmetric unit and diffract to about 1.9 A. X-ray data have been collected to 2.0 A from a native crystal. The previously published amino acid sequence of the protein has been corrected at positions 71, 72, 113, 114 and 156.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Englebert, S.

Charlier, Paulette ; Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques

Fonze, E.

To'th, Y.

Vermeire, M.

Van Beeumen, J.

Grandchamps, J.

Hoffmann, K.

Leyh-Bouille, M.

Nguyen-Distèche, Martine

Ghuysen, Jean-Marie

Language :

English

Title :

Crystallization and X-ray diffraction study of the Streptomyces K15 penicillin-binding DD-transpeptidase.

Publication date :

1994

Journal title :

Journal of Molecular Biology

ISSN :

0022-2836

eISSN :

1089-8638

Publisher :

Academic Press, London, United Kingdom

Volume :

241

Issue :

Pages :

295-7

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 November 2010

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Bibliography

Charlier, P., Buisson, G., Dideberg, O., Wierenga, J., Keck, W., Laible, G. & Hakenbeck, R. (1993). Crystallization of a genetically engineered water-soluble primary target enzyme. The high molecular mass PBP2X of Streptococcus pneumoniae. J. Mol. Biol. 232, 1007-1009.
Ferreira, L. C. S., Keck, W., Schwarz, U., Charlier, P., Dideberg, O. & Ghuysen, J. M. (1988). Construction, isolation, characterization and crystallization of an enzymatically active water-soluble PBP5 produced by Escherichia coli K12. Eur. J. Biochem. 171, 11-16.
Granier, B., Jamin, M., Adam, M., Galleni, M., Lakaye, B., Zorzi, W., Grandchamps, J., Wilkin, J. M., Fraipont, C., Joris, B., Duez, C., Nguyen-Distèche, M., Coyette, J., Leyh-Bouille, M., Dusart, J., Christiaens, L., Frère, J. M. & Ghuysen, J. M. (1994). Serine-type D-Ala-D-Ala peptidases and penicillin-binding proteins. Methods Enzymol. 244, chapt. 19.
Howard, A. J. (1991). XENGEN version 2.0 manual. Protein Engineering Department, Genex Corporation, Gaithersburg, MA, U.S.A.
Kelly, J. A., Knox, J. R., Zhao, H., Frère, J. M. & Ghuysen, J. M. (1989). Crystallographic mapping of β-lactams bound to a DD-peptidase target enzyme, J. Mol. Biol. 209, 281-295.
Leyh-Bouille, M., Nguyen-Distèche, M., Bellefroid-Bourguignon, C. & Ghuysen, J. M. (1987). Effects of thiol reagents on Streptomyces K15 DD-peptidase-catalysed reactions. Biochem. J. 241, 893-897.
Matthews, B. W. (1968). Solvent content of protein crystals. J. Mol. Biol. 33, 491-497.
Nguyen-Distèche, M., Leyh-Bouille, M., Pirlot, S., Frère, J. M. & Ghuysen, J. M. (1986). Streptomyces K15 DD-peptidase-catalysed reactions with ester and amide carbonyl donors. Biochem. J. 235, 167-176.
Palomeque-Messia, P., Englebert, S., Leyh-Bouille, M., Nguyen-Distèche, M., Duez, C., Houba, S., Dideberg, O., Van Beeumen, J. & Ghuysen, J. M. (1991). Amino acid sequence of the penicillin-binding protein/ DD-peptidase of Streptomyces K15. Biochem. J. 279, 223-230.
Palomeque-Messia, P., Quittre, V., Leyh-Bouille, M., Nguyen-Distèche, M., Gershater, G. J. L., Dacey, I. K., Dusart, J., Van Beeumen, J. & Ghuysen, J. M. (1992). Secretion by overexpression and purification of the water-soluble Streptomyces K15 DD-transpeptidase/penicillin-binding protein. Biochem. J. 288, 87-91.