Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Ruelle, Virginie; Elmoualij, Benaïssa; Zorzi, Willy; Zorzi, Danièle; Ledent, P.; Pierard, Olivier; Bonjean, Nicolas; De Pauw-Gillet, Marie-Claire; Heinen, Ernst; De Pauw, Edwin

doi:10.1007/3-540-26531-7_4

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Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Ruelle, Virginie; Elmoualij, Benaïssa; Zorzi, Willy et al.

2005 • In Lichtfouse, Eric; Schwarzbauer, Jan; Robert, Didier (Eds.) Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems

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https://hdl.handle.net/2268/77223

DOI
10.1007/3-540-26531-7_4

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Keywords :

MALDI-TOF MS; bacteria; biomarker; activated sludge; wastewater treatment; optimization

Abstract :

[en] In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil.

Research center :

CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
CRPP - Centre de Recherche sur les Protéines Prion - ULiège

Disciplines :

Environmental sciences & ecology
Chemistry

Author, co-author :

Ruelle, Virginie

Elmoualij, Benaïssa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie

Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques

Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Ledent, P.

Pierard, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Bonjean, Nicolas

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Language :

English

Title :

Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Publication date :

2005

Main work title :

Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems

Editor :

Lichtfouse, Eric

Schwarzbauer, Jan

Robert, Didier

Publisher :

Springer, Berlin, Germany

Pages :

31-42

Name of the research project :

I-MALDI-MS: Reconnaissance moléculaire d'agents microbiens par immuno-MALDI-MS

Funders :

DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]

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Bibliography

Anhalt JP, Fenselau C (1975) Identification of bacteria using mass spectrometry. Anal Chem 47:219-225.
Arnold RJ, Karty JA, Ellington AD, Reilly JP (1999) Monitoring the growth of bacteria culture by MALDI-MS of whole cells. Anal Chem 71:1990-1996.
Banks CJ, Walker I (1977) Sonication of activated sludge flocs and the recovery of their bacteria on solid media. J General Microbiol 98:363-368.
Birmingham J, Demirev P, Ho YP, Thomas J, Bryden W, Fenselau C (1999) Corona plasma discharge for rapid analysis of microorganisms by mass spectrometry. Rapid Commun Mass Spectrom 13:604-606.
Chong BE, Wall DB, Lubman DM, Flynn SJ (1997) Rapid profiling of E. coli proteins up to 500 kDa from whole cell lysates using MALDI-TOF MS. Rapid Commun Mass Spectrom 11:1900-1908.
Dai Y, Li L, Roser DC, Long SR (1999) Detection and identification of low-mass peptides and proteins from solvent suspensions of Escherichia coli by HPLC fractionation and MALDI-TOF MS. Rapid Commun Mass Spectrom 13:73-78.
Demirev PA, Ramirez J, Fenselau C (2001) Tandem mass spectrometry of intact proteins for characterization of biomarkers from Bacillus cereus T spores. Anal Chem 73:5725-5731.
Difco Laboratories (1998) Difco manual, 11th edn. Difco Laboratories, Division of Becton Dickinson and company, Sparks, Maryland, USA.
Domin MA, Welham KJ, Ashton DS (1999) The effect of solvent and matrix combinations on the analysis of bacteria by MALDI-TOF MS. Rapid Commun Mass Spectrom 13:222-226.
Easterling ML, Colangelo CM, Scott RA, Amster IJ (1998) Monitoring protein expression in whole bacterial cells with MALDI-TOF MS. Anal Chem 70:2704-2709.
Elhanany E, Barak R, Fisher M, Kobiler D, Altboum Z (2001) Detection of specific Bacillus anthracis spore biomarkers by MALDI-TOF MS. Rapid Commun Mass Spectrom 15:2110-2116.
Evason DJ, Claydon MA, Gordon DB (2001) Exploring the limits of bacterial identification by intact cell-mass spectrometry. J Am Soc Mass Spectrom 12:49-54.
Fenselau C, Demirev PA (2001) Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrometry Reviews 20:157-171.
Frolund B, Palmgren R, Keiding K, Nielsen P (1995) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30(8):1749-1758.
Haag AM, Taylor SN, Johnston KH, Cole RB (1998) Rapid identification and speciation of Haemophilus bacteria by MALDI-TOF MS. J Mass Spectrom 33:750-756.
Hamels S, Gala JL, Dufour S, Vannuffel P, Zammatteo N, Remacle J (2001) Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance. Bio Techn 31:1364-1372.
Holland RD, Wilkes JG, Rafii F, Sutherland JB, Persons CC, Voorhees KJ, Lay JO (1996) Rapid identification of intact whole bacteria based on spectral patterns using MALDI-TOF MS. Rapid Commun Mass Spectrom 10:1227-1232.
Holland RD, Duffy CR, Rafii F, Sutherland JB, Heinze TM, Holder CL, Voorhees KJ, Lay JO (1999) Identification of bacterial proteins observed in MALDI TOF mass spectra from whole cells. Anal Chem 71:3226-3230.
Jorand F, Zartarian F, Thomas F, Block JC, Bottero JY, Villemin G, Urbain V, Manen J (1995) Chemicals and structural (2D) linkage between bacteria within activated sludge flocs. Water Res 29(7):1639-1647.
Karas M, Bachmann D, Bahr U, Hillenkamp F (1987) Matrix-assisted ultraviolet laser desorption of non volatile compounds. Int J Mass Spectrom Ion Processes 78:53-68.
Krishnamurthy T, Ross PL, Rajamani U (1996) Detection of pathogenic and non-pathogenic bacteria by MALDI-TOF MS. Rapid Commun Mass Spectrom 10:883-888.
Krishnamurthy T, Davis MT, Stahl DC, Lee TD (1999) Liquid chromatography/microspray mass spectrometry for bacterial investigations. Rapid Commun Mass Spectrom 13:39-49.
Lynn EC, Chung MC, Tsai WC, Han CC (1999) Identification of Enterobacteriaceae bacteria by direct MALDI mass spectrometric analysis of whole cells. Rapid Commun Mass Spectrom 13:2022-2027.
Madonna AJ, Basile F, Ferrer I, Meetani MA, Rees JC, Voorhees KJ (2000) On-probe sample pretreatment for the detection of proteins above 15 kDa from whole cell bacteria by MALDI-TOF MS. Rapid Commun Mass Spectrom 14:2220-2229.
Nichols DS, McMeckin TA (2002) Biomarker techniques to screen for bacteria that proceduce polyunsaturated fatty acids. J Microbial Methods 48(2-3):161-170.
Nilsson CL (1999) Fingerprinting of Helicobacter pylori strains by MALDI mass spectrometric analysis. Rapid Commun Mass Spectrom 13:1067-1071.
Ryzhov V, Fenselau C (2001) Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal Chem 73:746-750.
Ryzhov V, Hathout Y, Fenselau C (2000) Rapid characterization of spores of Bacillus cereus group bacteria by MALDI-TOF MS. Appl Environ Microbiol 66(9):3828-3834.
Saenz AJ, Petersen CE, Valentine NB, Gantt SL, Jarman KH, Kingsley MT, Wahl KL (1999) Reproducibility of MALDI-TOF MS for replicate bacterial culture analysis. Rapid Commun Mass Spectrom 13:1580-1585.
Snyder AP, Thornton SN, Dworzanski JP, Meuzelaar HLC (1996) Detection of the picolinic acid biomarker in Bacillus spores using a potentially field-portable pyrolysis-gas chromatographyion mobility spectrometry system. Field Anal Chem & Techn 1:49-59.
Thomas JJ, Falk B, Fenselau C (1998) Viral characterization by direct analysis of capsid proteins. Anal Chem 70:3863-3867.
Wang Z, Russon L, Li L, Roser DC, Long SR (1998) Investigation of spectral reproducibility in direct analysis of bacteria proteins by MALDI-TOF MS. Rapid Commun Mass Spectrom 12:456-464.
Welham KJ, Domin MA, Johnson K, Jones L, Ashton DS (2000) Characterization of fungal spores by laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 14:307-310.