Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Ruelle, Virginie; Elmoualij, Benaïssa; Zorzi, Willy; Zorzi, Danièle; Ledent, P.; Pierard, Olivier; Bonjean, Nicolas; De Pauw-Gillet, Marie-Claire; Heinen, Ernst; De Pauw, Edwin

doi:10.1007/3-540-26531-7_4

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Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Ruelle, Virginie; Elmoualij, Benaïssa; Zorzi, Willy et al.

2005 • In Lichtfouse, Eric; Schwarzbauer, Jan; Robert, Didier (Eds.) Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems

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https://hdl.handle.net/2268/77223

DOI
10.1007/3-540-26531-7_4

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Keywords :

MALDI-TOF MS; bacteria; biomarker; activated sludge; wastewater treatment; optimization

Abstract :

[en] In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil.

Research Center/Unit :

CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
CRPP - Centre de Recherche sur les Protéines Prion - ULiège

Disciplines :

Environmental sciences & ecology
Chemistry

Author, co-author :

Ruelle, Virginie

Elmoualij, Benaïssa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie

Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques

Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Ledent, P.

Pierard, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Bonjean, Nicolas

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Language :

English

Title :

Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Publication date :

2005

Main work title :

Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems

Editor :

Lichtfouse, Eric

Schwarzbauer, Jan

Robert, Didier

Publisher :

Springer, Berlin, Germany

Pages :

31-42

Name of the research project :

I-MALDI-MS: Reconnaissance moléculaire d'agents microbiens par immuno-MALDI-MS

Funders :

DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie

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since 25 November 2010

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