The Bacillus licheniformis BlaP beta-lactamase as a model protein scaffold to study the insertion of protein fragments.

Bacillus/enzymology; Bacterial Proteins/chemistry/genetics; Chitin/metabolism; Enzyme Stability; Hexosaminidases/chemistry; Models, Molecular; Protein Denaturation; Protein Structure, Tertiary; Recombinant Fusion Proteins/chemistry; beta-Lactamases/chemistry/genetics

Abstract :

[en] Using genetic engineering technologies, the chitin-binding domain (ChBD) of the human macrophage chitotriosidase has been inserted into the host protein BlaP, a class A beta-lactamase produced by Bacillus licheniformis. The product of this construction behaved as a soluble chimeric protein that conserves both the capacity to bind chitin and to hydrolyze beta-lactam moiety. Here we describe the biochemical and biophysical properties of this protein (BlaPChBD). This work contributes to a better understanding of the reciprocal structural and functional effects of the insertion on the host protein scaffold and the heterologous structured protein fragments. The use of BlaP as a protein carrier represents an efficient approach to the functional study of heterologous protein fragments.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Vandevenne, Marylène ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Filée, Patrice ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Scarafone, Natacha ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines

Cloes, Benoit

Gaspard, Gilles

Yilmaz, Nursel

Dumoulin, Mireille ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines

François, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Language :

English

Title :

The Bacillus licheniformis BlaP beta-lactamase as a model protein scaffold to study the insertion of protein fragments.

Publication date :

2007

Journal title :

Protein Science: A Publication of the Protein Society

ISSN :

0961-8368

eISSN :

1469-896X

Publisher :

Cold Spring Harbor Laboratory Press, Woodbury, United States - New York

Volume :

Issue :

Pages :

2260-71

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 November 2010

Statistics

Number of views

152 (14 by ULiège)

Number of downloads

282 (9 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Abedi, M.R., Caponigro, G., and Kamb, A. 1998. Green fluorescent protein as a scaffold for intracellular presentation of peptides. Nucleic Acids Res. 26: 623-630.
Betton, J.M., Jacob, J.P., Hofnung, M., and Broome-Smith, J.K. 1997. Creating a bifunctional protein by insertion of β-lactamase into the maltodextrin-binding protein. Nat. Biotechnol. 15: 1276-1279.
Collinet, B., Herve, M., Pecorari, F., Minard, P., Eder, O., and Desmadril, M. 2000. Functionally accepted insertions of proteins within protein domains. J. Biol. Chem. 275: 17428-17433.
Delarue, M., Poch, O., Tordo, N., Moras, D., and Argos, P. 1990. An attempt to unify the structure of polymerases. Protein Eng. 3: 461-467.
Feller, G., d'Amico, D., and Gerday, C. 1999. Thermodynamic stability of a cold-active α-amylase from the Antarctic bacterium Alteromonas haloplanctis. Biochemistry 38: 4613-4619.
Frate, M.C., Lietz, E.J., Santos, J., Rossi, J.P., Fink, A.L., and Ermacora, M.R. 2000. Export and folding of signal-sequenceless Bacillus licheniformis β-lactamase in Escherichia coli. Eur. J. Biochem. 267: 3836-3847.
Freimuth, P.I., Taylor, J.W., and Kaiser, E.T. 1990. Introduction of guest peptides into Escherichia coli alkaline phosphatase. Excision and purification of a dynorphin analogue from an active chimeric protein. J. Biol. Chem. 265: 896-901.
Gilkes, N.R., Jervis, E., Henrissat, B., Tekant, B., Miller Jr., R.C., Warren, R.A., and Kilburn, D.G. 1992. The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose. J. Biol. Chem. 267: 6743-6749.
Hallet, B., Sherratt, D.J., and Hayes, F. 1997. Pentapeptide scanning mutagenesis: Random insertion of a variable five amino acid cassette in a target protein. Nucleic Acids Res. 25: 1866-1867.
Han, K.K., Tetaert, D., Debuire, B., Dautrevaux, M., and Biserte, G. 1977. Sequential Edman degredation. Biochimie 59: 557-576.
Hashimoto, M., Ikegami, T., Seino, S., Ohuchi, N., Fukada, H., Sugiyama, J., Shirakawa, M., and Watanabe, T. 2000. Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12. J. Bacteriol. 182: 3045-3054.
Hirabayashi, J. and Kasai, K. 1991. Effect of amino acid substitution by sited-directed mutagenesis on the carbohydrate recognition and stability of human 14-kDa β-galactoside-binding lectin. J. Biol. Chem. 266: 23648-23653.
Jones, S., Stewart, M., Michie, A., Swindells, M.B., Orengo, C., and Thornton, J.M. 1998. Domain assignment for protein structures using a consensus approach: Characterization and analysis. Protein Sci. 7: 233-242.
Ladant, D., Glaser, P., and Ullmann, A. 1992. Insertional mutagenesis of Bordetella pertussis adenylate cyclase. J. Biol. Chem. 267: 2244-2250.
Lee, C. and Levitt, M. 1991. Accurate prediction of the stability and activity effects of site-directed mutagenesis on a protein core. Nature 352: 448-451.
Levine, M., Muirhead, H., Stammers, D.K., and Stuart, D.I. 1978. Structure of pyruvate kinase and similarities with other enzymes: Possible implications for protein taxonomy and evolution. Nature 271: 626-630.
Lewis, J.K., Bendahmane, M., Smith, T.J., Beachy, R.N., and Siuzdak, G. 1998. Identification of viral mutants by mass spectrometry. Proc. Natl. Acad. Sci. 95: 8596-8601.
Martin, J.L., Bardwell, J.C., and Kuriyan, J. 1993. Crystal structure of the DsbA protein required for disulphide bond formation in vivo. Nature 365: 464-468.
Matagne, A., Misselyn-Bauduin, A.M., Joris, B., Erpicum, T., Granier, B., and Frere, J.M. 1990. The diversity of the catalytic properties of class A β-lactamases. Biochem. J. 265: 131-146.
Matagne, A., Joris, B., Van Beeumen, J., and Frere, J.M. 1991. Ragged N-termini and other variants of class A β-lactamases analysed by chromato-focusing. Biochem. J. 273: 503-510.
Mathonet, P., Deherve, J., Soumillion, P., and Fastrez, J. 2006. Active TEM-1 β-lactamase mutants with random peptides inserted in three contiguous surface loops. Protein Sci. 15: 2323-2334.
Moews, P.C., Knox, J.R., Dideberg, O., Charlier, P., and Frere, J.M. 1990. β-Lactamase of Bacillus licheniformis 749/C at 2 Å resolution. Proteins 7: 156-171.
Pace, C.N. 1986. Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol. 131: 266-280.
Palzkill, T. and Botstein, D. 1992. Probing β-lactamase structure and function using random replacement mutagenesis. Proteins 14: 29-44.
Prodromou, C. and Pearl, L.H. 1992. Recursive PCR: A novel technique for total gene synthesis. Protein Eng. 5: 827-829.
Rouse, D.A., DeVito, J.A., Li, Z., Byer, H., and Morris, S.L. 1996. Site-directed mutagenesis of the katG gene of Mycobacterium tuberculosis: Effects on catalase-peroxidase activities and isoniazid resistance. Mol. Microbiol. 22: 583-592.
Selwyn, M.J. 1965. A simple test for inactivation of an enzyme during assay. Biochim. Biophys. Acta 105: 193-195.
Sideraki, V., Huang, W., Palzkill, T., and Gilbert, H.F. 2001. A secondary drug resistance mutation of TEM-1 β-lactamase that suppresses misfolding and aggregation. Proc. Natl. Acad. Sci. 98: 283-288.
Tepper, A.D., Dammann, H., Bominaar, A.A., and Veron, M. 1994. Investigation of the active site and the conformational stability of nucleoside diphosphate kinase by site-directed mutagenesis. J. Biol. Chem. 269: 32175-32180.
Tjoelker, L.W., Gosting, L., Frey, S., Hunter, C.L., Trong, H.L., Steiner, B., Brammer, H., and Gray, P.W. 2000. Structural and functional definition of the human chitinase chitin-binding domain. J. Biol. Chem. 275: 514-520.
Tomme, P., Creagh, A.L., Kilburn, D.G., and Haynes, C.A. 1996. Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis. Biochemistry 35: 13885-13894.
Tsybovsky, Y.I., Shubenok, D.V., Stremovskiy, O.A., Deyev, S.M., and Martsev, S.P. 2004. Folding and stability of chimeric immunofusion VL-barstar. Biochemistry (Mosc.) 69: 939-948.
Ujita, M., Sakai, K., Hamazaki, K., Yoneda, M., Isomura, S., and Hara, A. 2003. Carbohydrate binding specificity of the recombinant chitin-binding domain of human macrophage chitinase. Biosci. Biotechnol. Biochem. 67: 2402-2407.