Article (Scientific journals)
Specificity and reversibility of the transpeptidation reaction catalyzed by the Streptomyces R61 D-Ala-D-Ala peptidase
Rhazi, Noureddine; Delmarcelle, Michaël; Sauvage, Eric et al.
2005In Protein Science: A Publication of the Protein Society, 14 (11), p. 2922-2928
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Keywords :
D-Ala-D-Ala peptidase; transpeptidation; reversibility; specificity; beta-lactam antibiotic; penicillin binding protein; enzymes; active sites; thermodynamics; hydrodynamics; kinetics; mechanism
Abstract :
[en] The specificity of the Streptomyces R61 penicillin-sensitive D-Ala-D-Ala peptidase has been re-examined with the help of synthetic substrates. The products of the transpeptidation reactions obtained with Gly-L-Xaa dipeptides as acceptor substrates are themselves poor substrates of the enzyme. This is in apparent contradiction with the classically accepted specificity rules for D-Ala-D-Ala peptidases. The Gly-L-Xaa dipeptide is regenerated by both the hydrolysis and transpeptidation reactions. The latter reaction is observed when another Gly-L-Xaa peptide or D-Alanine are supplied as acceptors. Utilization of substrates in which the terminal -COO(-) group has been esterified or amidated shows that a free carboxylate is not an absolute prerequisite for activity. The results are discussed in the context of the expected reversibility of the transpeptidation reaction.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rhazi, Noureddine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Delmarcelle, Michaël ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Sauvage, Eric ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Jacquemotte, Françoise;  Institut Meurice, Haute Ecole Lucia de Brouckère (Bruxelles, Belgium) > Département des Substances Naturelles et Biochimie
Devriendt, Kris;  Universiteit Gent > Laboratorium voor Eiwitbiochemie and Eiwitengineering
Tallon, Valérie;  Université Catholique de Louvain
Ghosez, Léon ;  Université de Liège - ULiège > Relations académiques et scientifiques (Sciences)
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Language :
English
Title :
Specificity and reversibility of the transpeptidation reaction catalyzed by the Streptomyces R61 D-Ala-D-Ala peptidase
Publication date :
November 2005
Journal title :
Protein Science: A Publication of the Protein Society
ISSN :
0961-8368
eISSN :
1469-896X
Publisher :
Cold Spring Harbor Lab Press, Publications Dept, Woodbury, United States - New York
Volume :
14
Issue :
11
Pages :
2922-2928
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 October 2010

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