Reference : Divergence of function and regulation of class B floral organ identity genes.
Scientific journals : Article
Life sciences : Genetics & genetic processes
Divergence of function and regulation of class B floral organ identity genes.
Samach, A. [> > > >]
Kohalmi, S. E. [> > > >]
Motte, Patrick mailto [Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Datla, R. [> > > >]
Haughn, G. W. [> > > >]
Plant Cell
American Society of Plant Biologists
Yes (verified by ORBi)
[en] Arabidopsis/genetics/ultrastructure ; Gene Expression Regulation, Plant ; Genes, Plant ; Microscopy, Electron, Scanning ; Phenotype ; Plants, Genetically Modified ; RNA, Messenger/genetics
[en] Regulatory mechanisms controlling basic aspects of floral morphogenesis seem to be highly conserved among plant species. The class B organ identity genes, which are required to establish the identity of organs in the second (petals) and third (stamens) floral whorls, are a good example of such conservation. This work compares the function of two similar class B genes in the same genetic background. The DEFICIENS (DEF) gene from Antirrhinum, including its promoter, was transformed into Arabidopsis and compared in function and expression with the Arabidopsis class B genes APETALA3 (AP3) and PISTILLATA (PI). The DEF gene was expressed in the second, third, and fourth whorls, as was PI. Functionally, DEF could replace AP3 in making petals and stamens. The DEF gene's AP3-like function and PI-like expression caused transformation of fourth-whorl carpels to stamens. Like AP3, all aspects of DEF function in Arabidopsis required a functional PI protein. Surprisingly, DEF could not replace the AP3 protein in properly maintaining AP3 transcripts (autoregulation). Our data allow us to revise the current model for class B autoregulation and propose a hypothesis for the evolution of class B gene expression in dicotyledonous plants.
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