Divergence of function and regulation of class B floral organ identity genes.

Arabidopsis/genetics/ultrastructure; Gene Expression Regulation, Plant; Genes, Plant; Microscopy, Electron, Scanning; Phenotype; Plants, Genetically Modified; RNA, Messenger/genetics

Abstract :

[en] Regulatory mechanisms controlling basic aspects of floral morphogenesis seem to be highly conserved among plant species. The class B organ identity genes, which are required to establish the identity of organs in the second (petals) and third (stamens) floral whorls, are a good example of such conservation. This work compares the function of two similar class B genes in the same genetic background. The DEFICIENS (DEF) gene from Antirrhinum, including its promoter, was transformed into Arabidopsis and compared in function and expression with the Arabidopsis class B genes APETALA3 (AP3) and PISTILLATA (PI). The DEF gene was expressed in the second, third, and fourth whorls, as was PI. Functionally, DEF could replace AP3 in making petals and stamens. The DEF gene's AP3-like function and PI-like expression caused transformation of fourth-whorl carpels to stamens. Like AP3, all aspects of DEF function in Arabidopsis required a functional PI protein. Surprisingly, DEF could not replace the AP3 protein in properly maintaining AP3 transcripts (autoregulation). Our data allow us to revise the current model for class B autoregulation and propose a hypothesis for the evolution of class B gene expression in dicotyledonous plants.

Disciplines :

Genetics & genetic processes

Author, co-author :

Samach, A.

Kohalmi, S. E.

Motte, Patrick ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Datla, R.

Haughn, G. W.

Language :

English

Title :

Divergence of function and regulation of class B floral organ identity genes.

Publication date :

1997

Journal title :

Plant Cell

ISSN :

1040-4651

eISSN :

1532-298X

Publisher :

American Society of Plant Biologists, Rockville, United States - Maryland

Volume :

Issue :

Pages :

559-70

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 September 2010

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