Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in an in vitro model of preeclamptic vascular endothelium: a possible therapeutic role of nicotinic acetylcholine receptor (nAChR) agonists for preeclampsia.
Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in an in vivo model of preeclamptic vascular endothelium.pdf
Author postprint (394.63 kB)
This is an electronic version (Author’s postprint) of an article published in American Journal of Obstetrics and Gynecology; 2010 May;202(5):464.e1-6. The original published version is available at: http://www.ajog.org/article/S0002-9378(10)00067-0/pdf
[en] OBJECTIVE: In this study we tested the hypothesis that nicotine restores proangiogenic functions to endothelial cells pretreated with soluble fms-like tyrosine kinase 1 and/or soluble endoglin. STUDY DESIGN: Wound healing assay and tube formation assay were performed using human umbilical vein endothelial cells treated with nicotine (10(-9) to 10(-6) M), and with various combinations of soluble fms-like tyrosine kinase 1 (100 ng/mL), soluble endoglin (100 ng/mL), and nicotine (10(-7) M). Enzyme-linked immunosorbent assay was performed to measure vascular endothelial growth factor, placental growth factor, and transforming growth factor-beta1 concentrations in the conditioned media treated with nicotine (10(-9) to 10(-6) M). RESULTS: Nicotine significantly facilitated endothelial migration and tube formation. By contrast, soluble fms-like tyrosine kinase 1 and/or soluble endoglin suppressed these endothelial functions. Nicotine restored these soluble fms-like tyrosine kinase 1 and/or soluble endoglin-reduced endothelial functions. Placental growth factor, but not transforming growth factor-beta1, production was significantly stimulated by the presence of nicotine. Vascular endothelial growth factor was undetectable. CONCLUSION: Our results suggest a possible mechanism for the protective effects of cigarette smoking against preeclampsia, thus proposing a therapeutic potential of nicotine or other nicotinic acetylcholine receptor agonists for preeclampsia.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Mimura, Kazuya; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Tomimatsu, Takuji; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Sharentuya, Namuxila; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Tskitishvili, Ekaterine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement > Osaka University Graduate School of Medicine, Department of Obstetrics and Gynecology
Kinugasa-Taniguchi, Yukiko; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Kanagawa, Takeshi; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Kimura, Tadashi; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Language :
English
Title :
Nicotine restores endothelial dysfunction caused by excess sFlt1 and sEng in an in vitro model of preeclamptic vascular endothelium: a possible therapeutic role of nicotinic acetylcholine receptor (nAChR) agonists for preeclampsia.
Publication date :
2010
Journal title :
American Journal of Obstetrics and Gynecology
ISSN :
0002-9378
eISSN :
1097-6868
Publisher :
Mosby, St Louis, United States - Missouri
Volume :
202
Issue :
5
Pages :
464.e1-6
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2010 Mosby, Inc. All rights reserved.
Sibai B., Dekker G., and Kupferminc M. Pre-eclampsia. Lancet 365 (2005) 785-799
Redman C.W., and Sargent I.L. Latest advances in understanding preeclampsia. Science 308 (2005) 1592-1594
Robertson W.B., Brosens I., and Dixon H.G. The pathological response of the vessels of the placental bed to hypertensive pregnancy. J Pathol Bacteriol 93 (1967) 581-592
Roberts J.M. Endothelial dysfunction in preeclampsia. Semin Reprod Endocrinol 16 (1998) 5-15
Yuan H.T., Haig D., and Karumanchi S.A. Angiogenic factors in the pathogenesis of preeclampsia. Curr Top Dev Biol 71 (2005) 297-312
Levine R.J., Maynard S.E., Qian C., et al. Circulating angiogenic factors and the risk of preeclampsia. N Engl J Med 350 (2004) 672-683
Levine R.J., Thadhani R., Qian C., et al. Urinary placental growth factor and risk of preeclampsia. JAMA 293 (2005) 77-85
Levine R.J., Lam C., Qian C., et al. Soluble endoglin and other circulating antiangiogenic factors in preeclampsia. N Engl J Med 355 (2006) 992-1005
Chaiworapongsa T., Romero R., Kim Y.M., et al. Plasma soluble vascular endothelial growth factor receptor-1 concentration is elevated prior to the clinical diagnosis of pre-eclampsia. J Matern Fetal Neonatal Med 17 (2005) 3-18
Hertig A., Berkane N., Lefevre G., et al. Maternal serum sFlt1 concentration is an early and reliable predictive marker of preeclampsia. Clin Chem 50 (2004) 1702-1703
Maynard S.E., Min J.Y., Merchan J., et al. Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J Clin Invest 111 (2003) 649-658
Koga K., Osuga Y., Yoshino O., et al. Elevated serum soluble vascular endothelial growth factor receptor 1 (sVEGFR-1) levels in women with preeclampsia. J Clin Endocrinol Metab 88 (2003) 2348-2351
Chaiworapongsa T., Romero R., Espinoza J., et al. Evidence supporting a role for blockade of the vascular endothelial growth factor system in the pathophysiology of preeclampsia. Am J Obstet Gynecol 190 (2004) 1541-1547
Powers R.W., Roberts J.M., Cooper K.M., et al. Maternal serum soluble fms-like tyrosine kinase 1 concentrations are not increased in early pregnancy and decrease more slowly postpartum in women who develop preeclampsia. Am J Obstet Gynecol 193 (2005) 185-191
Kendall R.L., Wang G., and Thomas K.A. Identification of a natural soluble form of the vascular endothelial growth factor receptor, FLT-1, and its heterodimerization with KDR. Biochem Biophys Res Commun 226 (1996) 324-328
Venkatesha S., Toporsian M., Lam C., et al. Soluble endoglin contributes to the pathogenesis of preeclampsia. Nat Med 12 (2006) 642-649
Toporsian M., Gros R., Kabir M.G., et al. A role for endoglin in coupling eNOS activity and regulating vascular tone revealed in hereditary hemorrhagic telangiectasia. Circ Res 96 (2005) 684-692
Bdolah Y., Sukhatme V.P., and Karumanchi S.A. Angiogenic imbalance in the pathophysiology of preeclampsia: newer insights. Semin Nephrol 24 (2004) 548-556
Conde-Agudelo A., Althabe F., Belizán J.M., and Kafury-Goeta A.C. Cigarette smoking during pregnancy and risk of preeclampsia: a systematic review. Am J Obstet Gynecol 181 (1999) 1026-1035
Hammoud A.O., Bujold E., Sorokin Y., Schild C., Krapp M., and Baumann P. Smoking in pregnancy revisited: findings from a large population-based study. Am J Obstet Gynecol 192 (2005) 1856-1862
Jeyabalan A., Powers R.W., Durica A.R., Harger G.F., Roberts J.M., and Ness R.B. Cigarette smoke exposure and angiogenic factors in pregnancy and preeclampsia. Am J Hypertens 21 (2008) 943-947
Heeschen C., Jang J.J., Weis M., et al. Nicotine stimulates angiogenesis and promotes tumor growth and atherosclerosis. Nat Med 7 (2001) 833-839
Cooke J.P. Angiogenesis and the role of the endothelial nicotinic acetylcholine receptor. Life Sciences 80 (2007) 2347-2351
Darby T.D., McNamee J.E., and van Rossum J.M. Cigarette smoking pharmacokinetics and its relationship to smoking behavior. Clin Pharmacokinet 9 (1984) 435-449
Hill P., Haley N.J., and Wynder E.L. Cigarette smoking: carboxyhemoglobin, plasman nicotine, cotinine and thiocyanate vs self-reported smoking data and cardiovascular disease. J Chronic Dis 36 (1983) 439-449
Jain R.K. Clearing the smoke on nicotine and angiogenesis. Nat Med 7 (2001) 775-777
Conklin B.S., Zhao W., Zhong D.S., and Chen C. Nicotine and cotinine up-regulate vascular endothelial growth factor expression in endothelial cells. Am J Pathol 160 (2002) 413-418
Heeschen C., Weis M., and Cooke J.P. A novel angiogenic pathway mediated by non-neuronal nicotinic acetylcholine receptors. J Clin Invest 110 (2002) 527-536
Goodwin A.M. In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents. Microvasc Res 74 (2007) 172-183
Frick M., Dulak J., Cisowski J., et al. Statins differentially regulate vascular endothelial growth factor synthesis in endothelial and vascular smooth muscle cells. Atherosclerosis 170 (2003) 229-236
Imaizumi T., Itaya H., Nasu S., et al. Expression of vascular endothelial growth factor in human umbilical vein endothelial cells stimulated with interleukin-1alpha-an autocrine regulation of angiogenesis and inflammatory reactions. Thromb Haemost 83 (2000) 949-955
Ogburn P.L., Hurt R.D., Croghan I.T., et al. Nicotine patch use in pregnant smokers: nicotine and cotinine levels and fetal effects. Am J Obset Gynecol 181 (1999) 736-743
Wisborg K., Henriksen T.B., Jespersen L.B., and Secher N.J. Nicotine patches for pregnant smokers: a randomized controlled study. Obstet Gynecol 96 (2000) 967-971
Oncken C., Dornelas E., Greene J., et al. Nicotine gum for pregnant smokers: a randomized controlled trial. Obstet Gynecol 112 (2008) 859-867
Ernst M., Moolchan E.T., and Robinson M.L. Behavioral and neural consequences of prenatal exposure to nicotine. J Am Acad Child Adolesc Psychiatry 40 (2001) 630-641
Jonge W., and Ulloa L. The alpha7 nicotinic acetylcholine receptor as a pharmacological target for inflammation. Br J Pathol 151 (2007) 915-929
Bainbridge S.A., Farley A.E., McLaughlin B.E., et al. Carbon monoxide decreases perfusion pressure in isolated human placenta. Placenta 23 (2002) 563-569
England L.J., Levine R.J., Mills J.L., Klebanoff M.A., Yu K.F., and Cnattingius S. Adverse pregnancy outcomes in snuff users. Am J Obstet Gynecol 189 (2003) 939-943
Bainbridge S.A., Belkacemi L., Dickinson M., Graham C.H., and Smith G.N. Carbon monoxide inhibits hypoxia/reoxygenation-induced apoptosis and secondary necrosis in syncytiotrophoblast. Am J Pathol 169 (2006) 774-783
Baum M., Schiff E., Kreiser D., et al. End-tidal carbon monoxide measurements in women with pregnancy-induced hypertension and preeclampsia. Am J Obstet Gynecol 183 (2000) 900-903
Kreiser D., Baum M., Seidman D.S., et al. End tidal carbon monoxide levels are lower in women with gestational hypertension and pre-eclampsia. J Perinatol 24 (2004) 213-217
Cudmore M., Ahmad S., Al-Ani B., et al. Negative regulation of soluble Flt-1 and soluble endoglin release by heme oxygenase-1. Circulation 115 (2007) 1789-1797
Dowling O., Rochelson B., Way K., Al-Abed Y., and Metz C.N. Nicotine inhibits cytokine production by placenta cells via NFkappaB: potential role in pregnancy-induced hypertension. Mol Med 13 (2007) 576-583
Mehendale R., Hibbard J., Fazleabas A., and Leach R. Placental angiogenesis markers sFlt-1 and PlGF: response to cigarette smoke. Am J Obstet Gynecol 197 (2007) 363.e1-363.e5
Ahmad S., and Ahmed A. Antiangiogenic effect of soluble vascular endothelial growth factor receptor-1 in placental angiogenesis. Endothelium 12 (2005) 89-95
Fisher S.J. The placental problem: linking abnormal cytotrophoblast differentiation to the maternal symptoms of preeclampsia. Reprod Biol Endocrinol 2 (2004) 53
Zhou Y., McMaster M., Woo K., et al. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Am J Pathol 160 (2002) 1405-1423
Athanassiades A., and Lala P.K. Role of placenta growth factor (PIGF) in human extravillous trophoblast proliferation, migration and invasiveness. Placenta 19 (1998) 465-473
Desai J., Holt-Shore V., Torry R.J., Caudle M.R., and Torry D.S. Signal transduction and biological function of placenta growth factor in primary human trophoblast. Biol Reprod 60 (1999) 887-892
Wang H., Yu M., Ochani M., et al. Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 421 (2003) 384-388
Wang H., Liao H., Ochani M., et al. Cholinergic agonists inhibit HMGB1 release and improve survival in experimental sepsis. Nat Med 10 (2004) 1216-1221
Saeed R.W., Varma S., Peng-Nemeroff T., et al. Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation. J Exp Med 201 (2005) 1113-1123
Speer P., Zhang Y., Gu Y., Lucas M.J., and Wang Y. Effects of nicotine on intercellular adhesion molecule expression in endothelial cells and integrin expression in neutrophils in vitro. Am J Obstet Gynecol 186 (2002) 551-556
Borzychowski A.M., Sargent I.L., and Redman C.W. Inflammation and pre-eclampsia. Semin Fetal Neonatal Med 11 (2006) 309-316