ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes

[en] A new way to study the action of cyclodextrin was developed to quantify the damage caused on cell membrane and lipid bilayer. The Electron Spin Resonance (ESR) spectroscopy was used to study the action of Randomly methylated-beta-cyclodextrin (Rameb) on living cells (HCT-116). The relative anisotropy observed in ESR spectrum of nitroxide spin probe (5-DSA and cholestane) is directly related to the rotational mobility of the probe,which can be further correlated with themicroviscosity. The use of ESR probes clearly shows a close correlation between cholesterol contained in cells and cellular membrane microviscosity. This study also demonstrates the Rameb ability to extract cholesterol and phospholipids in time- and dose-dependent ways. In addition, ESR spectra enabled to establish that cholesterol is extracted from lipid rafts to form stable aggregates. The present work supports that ESR is an easy, reproducible and noninvasive technique to study the effect of cyclodextrins on cell membranes.

Disciplines :

Physics

Author, co-author :

Grammenos, Angeliki ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Guelluy, Pierre-Henri ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale

Lismont, Marjorie ; Université de Liège - ULiège > Département de physique > Biophotonique

Piel, Géraldine ; Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique

Hoebeke, Maryse ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale

Language :

English

Title :

ESR technique for non invasive way to quantify cyclodextrins effect on cell membranes

Publication date :

24 June 2010

Journal title :

Biochemical and Biophysical Research Communications

ISSN :

0006-291X

eISSN :

1090-2104

Publisher :

Academic Press, San Diego, United States - California

Pages :

1-5

Peer reviewed :

Peer reviewed

Available on ORBi :

since 30 July 2010

Statistics

Number of views

143 (30 by ULiège)

Number of downloads

421 (7 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Loftsson T., Brewster M.E. Pharmaceutical application of cyclodextrins. 1. Drug solubilisation and stabilization. J. Pharm. Sci. 1996, 85:1017-1024.
Loftsson T. Cyclodextrin in skin delivery. Cosmet. Toiletries 2000, 115(10):59-66.
Boulmedarat L., Piel G., Bochot A., Lesieur S., Delattre L., Fattal E. Cyclodextrin-mediated drug release from liposomes dispersed within a bioadhesive gel. Pharm. Res. 2005, 22:962-970.
Piel G., Moutard S., Uhoda E., Pilard F., Piérard G.E., Perly B., Delattre L., Evrard B. Skin compatibility of cyclodextrins and their derivative: a comparative assessment using a corneoxenometry bioassay. Eur. J. Pharm. Biopharm. 2004, 57:479-482.
Simons K., Ikonen E. Functional rafts in cell membranes. Nature 1997, 387:569-572.
Zajchowski L.D., Robbins S.M. Lipid rafts and little caves. Eur. J. Biochem. 2002, 269:737-752.
Subczynski W.K., Kusumi A. Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labeling and single molecule optical microscopy. Biochem. Biophys. Acta 2003, 1610:231-243.
Rivas M.G., Gennarlo A.M. Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study. Chem. Phys. Lipids 2003, 122:165-169.
Kawasaki K., Subczynski W.K., Hyde J.S., Kusumi A. Pulse EPR detection of lipid exchange between protein-rich raft and bulk domains in the membrane: methodology development and its application to studies of influenza viral membrane. Biophys. J. 2001, 80:738-748.
Singer S.J., Nicholson GL. The fluid mosaic model of the structure of cell membranes. Sciences 1972, 175:720-731.
Hopper N.M. Detergent-insoluble glycosphingolipid/cholesterol-rich membrane domains, lipid rafts and caveolae. Mol. Membr. Biol. 1999, 16:145-156.
Piel G., Piette M., Barillaro V., Castagne D., Evrard B., Delattre L. Study of the relationship between lipid binding properties of cyclodextrin and their effect on the integrity of liposomes. Int. J. Pharm. 2007, 338:35-42.
Castagne D., Fillet M., Delattre L., Evrard B., Nusgens B., Piel G. Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, relationships with their effects on endothelial cell viability and on membrane models. J. Incl. Phenom. Macrocycl. Chem. 2008, 63:225-231.
Zidovetzki R., Levitan I. Use of cyclodextrins to manipulate plasma membrane cholesterol content: evidence, misconceptions and control strategies. Biochim. Biophys. Acta 2007, 1768:1311-1324.
Witold K., Subczynski, Kusumi A. Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labelling and single optical microscopy. Biochim. Biophys. Acta 2003, 1610:231-243.
Suttmann H., Retz M., Paulsen F., Harder J., Zwergel U., Kamradt J., Wullich B., Unteregger G., Stöckle M., Lehmann J. Antimicrobial peptides of the Cecropin-family show potent antitumor activity against bladder cancer cells. BMC Urol. 2008, 8:1-7.
Grammenos A., Bahri M.A., Guelluy P.H., Piel G., Hoebeke M. Quantification of Randomly-methylated-β-cyclodextrin effect on liposome: an ESR study. Biochem. Biophys. Res. Commun. 2009, 390:5-9.
Piel G., Piette M., Barillaro V., Castagne D., Evrard B., Delattre L. Betamethasone-in-cyclodextrin-in-liposomes: the effect of cyclodextrins on encapsulation efficiency and release kinetics. Int. J. Pharm. 2006, 312:75-82.
Coderch L., Fonollosa J., De Pera M., Estelrich J., De La Maza A., Parra J.L. Influence of cholesterol on liposome fluidity by EPR relationship with percutaneous absorption. J. Controlled Release 2000, 68:85-95.
Olson F., Hunt C.A., Szoka F.C., Vail W.J., Papahadjopoulos D. Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes. Biochim. Biophys. Acta 1979, 557:9-23.
Arsov Z., Strancar J. Determination of partition coefficient of spin probe between different lipid membrane phases. J. Chem. Inf. Model. 2005, 45:1662-1667.
Arsov Z., Schara M., Zorko M., Strancar J. The membrane lateral domain approach in the studies of lipid-protein interaction of GPI-anchored bovine erythrocyte acetylcholinesterase. Eur. Biophys. J. 2004, 33:715-725.
McConnell H.M., Hubell W.L. Molecular motion in spin-labeled phospholipids and membranes. J. Am. Chem. Soc. 1971, 93:314-326.
Deo N.S.P. Electron spin resonance study of phosphatidylcholine vesicles using 5-doxyl stearic acid. Colloids Surf. B 2002, 25:225-232.
Bahri M.A., Heyne B.J., Hans P., Seret A.E., Mouithys-Mickalad A.A., Hoebeke M.D. Quantification of lipid bilayer effective microviscosity and fluidity effect induced by propofol. Biophys. Chem. 2005, 114:53-61.
Coderch L., Fonollosa J., De para M., Estelrich J., De La Maza A., Parra J.L. Influence of cholesterol on liposome fluidity by EPR relationship with percutaneous absorption. J. Controlled Release 2000, 68:85-95.
Mileo E., Franchi P., Gotti R., Bendazzoli C., Mezzina E., Lucarini M. An EPR method for measuring the rate of distribution of organic substrates between cyclodextrin, micelles and water. Chem. Commun. 2008, 1311-1313.
Bahri M.A., Hoebeke M., Grammenos A., Delanaye L., Vandewalle N., Seret A. Investigation of SDS, DTAB and CTAB micelle microviscosities by electron spin resonance. Colloids Surf. A 2006, 290:206-212.
Weglarz L., Koceva-Chyla A., Gwodzdzinski K., Dzierzewics Z., Jozwiak Z. Evaluation of hydralazine and procainamide effects on fibroblast. Biochem. J. 2003, 85:549-556.
Nguyen D.H., Taub D. Cholesterol is essential for macrophage inflammatory protein 1b binding and conformation integrity of CC chemokine receptor 5. Blood 2002, 12:4298-4360.