Metabolism of no-carrier-added 2-[18F]fluoro-L-tyrosine in rats

[en] Background: Several fluorine-18 labelled fluoroamino acids have been evaluated as tracers for the quantitative assessment of cerebral protein synthesis in vivo by positron emission tomography (PET). Among these, 2-[18F]fluoro-L-tyrosine (2-[18F]Tyr) has been studied in mice at a low specific activity. Its incorporation into proteins is fast and metabolism via other pathways is limited. The present in vivo study was carried out in normal awake rats using no-carrier-added 2-[18F]Tyr. Under normal physiological conditions, we have studied the incorporation into proteins and the metabolism of the tracer in different brain areas. Methods: No-carrier-added 2-[18F]Tyr was administered to awake rats equipped with chronic arterial and venous catheters. The time course of the plasma activity was studied by arterial blood sampling. The biodistribution of the activity in the main organs was studied at the end of the experiment. The distribution of radioactive species in plasma and brain regions was studied by acidic precipitation of the proteins and HPLC analysis of the supernatant. Results: The absolute uptake of radioactivity in brain regions was homogenous. In awake rats, nocarrier-added 2-[18F]Tyr exhibits a fast and almost quantitative incorporation into the proteins fractions of cerebellum and cortex. In striatum, this incorporation into proteins and the unchanged fraction of the tracer detected by HPLC could be lower than in other brain regions. Conclusion: This study confirms the potential of 2-[18F]fluoro-L-tyrosine as a tracer for the assessment of the rate of protein synthesis by positron emission tomography. The observed metabolism suggests a need for a correction for the appearance of metabolites, at least in plasma.

Research center :

GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Aerts, Joël ; Université de Liège - ULiège > Centre de recherches du cyclotron

Plenevaux, Alain ; Université de Liège - ULiège > Centre de recherches du cyclotron

Lemaire, Christian ; Université de Liège - ULiège > Centre de recherches du cyclotron

Giacomelli, Fabrice ; Université de Liège - ULiège > Centre de recherches du cyclotron

Warnock, Geoffrey ; Université de Liège - ULiège > Centre de recherches du cyclotron

Phillips, Christophe ; Université de Liège - ULiège > Centre de recherches du cyclotron

Luxen, André ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron

Language :

English

Title :

Metabolism of no-carrier-added 2-[18F]fluoro-L-tyrosine in rats

Publication date :

07 November 2008

Journal title :

BMC Medical Physics

eISSN :

1756-6649

Publisher :

BioMed Central, United Kingdom

Volume :

Pages :

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.biomedcentral.com/1756-6649/8/4

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 20 July 2010

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