Date palm, Sap, Phoenix dactylifera L.; SDS–PAGE, Mass spectrometry; Proteins
Résumé :
[en] The proteins contained in juice tapped from date palm (Phoenix dactylifera L.), from Deglet Nour variety,
were analysed by the application of two-dimensional electrophoresis (2DE). Identification was carried
out by mass spectrometry analyses. The SDS–PAGE patterns showed more than 100 spots of which 52
spots were identified. A proportion of the identified proteins were related to Saccharomyces cerevisiae that
may belong to the natural microflora of date palm sap. These proteins are principally involved in glycolysis.
While other proteins were assigned to be vegetable proteins, probably a mixture of proteins from the
vascular system, which have several biological functions within the palm tree. Thus, we found enzymes
involved in stress and defence reactions, in glycolysis, and photosynthesis reactions. Other enzymes are
associated with carbohydrates and proteins metabolisms.
Disciplines :
Biotechnologie
Auteur, co-auteur :
Ben Thabet, Imène
Francis, Frédéric ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Besbes, Souhail
Attia, Hamadi
Deroanne, Claude
Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.
Langue du document :
Anglais
Titre :
Characterisation of proteins from date palm sap (Phoenix dactylifera L.) by a proteomic approach
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