Characterisation of proteins from date palm sap (Phoenix dactylifera L.) by a proteomic  approach

[en] The proteins contained in juice tapped from date palm (Phoenix dactylifera L.), from Deglet Nour variety, were analysed by the application of two-dimensional electrophoresis (2DE). Identification was carried out by mass spectrometry analyses. The SDS–PAGE patterns showed more than 100 spots of which 52 spots were identified. A proportion of the identified proteins were related to Saccharomyces cerevisiae that may belong to the natural microflora of date palm sap. These proteins are principally involved in glycolysis. While other proteins were assigned to be vegetable proteins, probably a mixture of proteins from the vascular system, which have several biological functions within the palm tree. Thus, we found enzymes involved in stress and defence reactions, in glycolysis, and photosynthesis reactions. Other enzymes are associated with carbohydrates and proteins metabolisms.

Disciplines :

Biotechnology

Author, co-author :

Ben Thabet, Imène

Francis, Frédéric ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Besbes, Souhail

Attia, Hamadi

Deroanne, Claude

Blecker, Christophe ; Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.

Language :

English

Title :

Characterisation of proteins from date palm sap (Phoenix dactylifera L.) by a proteomic approach

Publication date :

01 May 2010

Journal title :

Food Chemistry

ISSN :

0308-8146

eISSN :

1873-7072

Publisher :

Elsevier Science, Oxford, United Kingdom

Pages :

765-770

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 June 2010

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