The definitive version is available at www3.interscience.wiley.com Publisher's version/PDF cannot be used author can archive pre-print author can archive post-print
All documents in ORBi are protected by a user license.
[en] Increased numbers of CD4+ Thy-1- cells have been described in the spleen (SP) of mice with retrovirus-induced immunodeficiency (MAIDS). Since this phenotypic abnormality might have considerable functional importance, the expansion of the CD4+ Thy-1- subset in MAIDS was characterized further. CD4+ Thy-1- and Thy-1+ T-cells from infected mice expressed similar densities of CD3 and TCR alpha/beta. In contrast, the Thy-1- subset was uniformly CD44hi, even early in the disease when part of Thy-1+ cells were still CD44lo. The emergence of CD4+ Thy-1- cells occurred first in SP and lymph nodes and was observed later in thymus. The important fraction of CD4+ cells lacking Thy-1 normally present in Peyer's patches was only weakly modified. Despite the major expansion of the CD4+ Thy-1- phenotype, the proliferating fraction was not higher in this subset than in CD4+ Thy-1+ cells from infected mice. Persistence after hydroxyurea administration was identical in both subsets, indicating similar mean cell lifespans. Taken together, these results show that the major expansion of CD4+ Thy-1- T-cells in MAIDS is not ascribable solely to increased proliferation within this subset. Phenotypic analysis suggests that CD4+ Thy-1- cells result from the differentiation of Thy-1+ cells induced by activation signals related to retroviral infection.
Disciplines :
Immunology & infectious disease
Author, co-author :
Moutschen, Michel ; Centre Hospitalier Universitaire de Liège - CHU > Maladies infectieuses et médecine interne générale
Colombi, S.; University of Liege, Liege, Belgium > Department of Pathology.
Deprez, Manuel ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Van Wijk, F.; Centre Hospitalier Universitaire de Liège - CHU > Department of Pathology.
Hotermans, C.; Centre Hospitalier Universitaire de Liège - CHU > Department of Pathology.
Martin, Michel
Greimers, Roland ; Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique
Boniver, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique
Language :
English
Title :
Population dynamics of CD4+ T cells lacking Thy-1 in murine retrovirus-induced immunodeficiency syndrome (MAIDS).
Mistry PB, Duplan JF (1973) Propriétés biologiques d'un virus isolé d'une radioleucémie C57BL. I. Premiers passages du virus natif. Bull Cancer 60:287-300.
Legrand E, Daculsi R, Duplan JF (1981) Characteristics of the cell populations involved in extra‐thymic lymphosarcoma induced in C57BL/6 mice by RadLV‐Rs. Leukemia Res 5:223-233.
Haas M, Resheef T (1980) Non‐thymic malignant lymphomas induced in C57BL/6 mice by cloned dualtropic viruses isolated from hematopoietic stromal cell lines. Eur J Cancer 16:909-917.
Mosier DE, Yetter RA, Morse HC (1985) Retroviral induction of acute lymphoproliferativ e disease and profound immunosuppression in adult C57/BL6 mice. J Exp Med 161:766-784.
Morse HC, Yetter RA, Via CS (1989) Functional and phenotypic alterations in T cell subsets during the course of MAIDS, a murine reirovirus‐induced immunodeficiency syndrome. J Immunol 143:844-850.
Cerny A, Hügin AW, Holmes K, Morse Ml HC (1990) CD4+ T cells in murine acquired immunodeficiency syndrome: evidence for an intrinsic defect in the proliferative response to soluble antigen. Eur J Immunol 20:1577-1581.
Fitzpatrick EA, Bryson JS, Rhoads C, Kaplan AM, Cohen DA (1992) T‐deficient transmembrane signaling in CD4+ T cells of retroviral‐induced immimc‐delkient mice. J Immunol 148:3337-3384.
Chattopadhyay SK, Morse HC, Makino M, Ruscetti SK, Hartley JW (1989) Defective retrovirus is associated with induction of murine retrovirus‐induced immunodeficiency syndrome. Proc Natl Acad Sci USA 86:3862-3866.
Aziz DC, Hanna Z, Jolicoeur P (1989) Severe immunodeficiency induced by a defective murine leukemia virus. Nature 338:505-508.
Huang M, Simard C, Jolicoeur P (1989) Immunodeficiency and clonal growth of target cells induced by helper‐free defective retrovirus. Science 246:1614-1617.
HuLing M, Simard C, Kay DG, Jolicoeur P (1991) The majority of cells infected with the defective murine AIDS virus belong to the B‐cell lineage. J Virol 65:6562-6571.
Jolicoeur P (1991) Murine acquired immunodeficiency syndrome (MAIDS): an animal model to study the AIDS pathogenesis. FASEB J 5:2398-2405.
Gazzinelli RT, Makino M, Chattopadhyay SK, Snapper CM, Sher A, Hugin AW, Morse HC (1992) CD4+ subset regulation in viral infection. Preferenlial activation of T h2 cells during progression of retrovirus‐induced immunodeficiency in mice. J Immunol 148:182-188.
Hiigin AW, Vacchio MS, Morse HC (1991) A virus‐encoded ‘superantigen’ in a retrovirus‐induced immunodeficiency syndrome of mice. Science 252:424-426.
Kanagawa O, Nussrallah BA, Wiebanga ME, Murphy KM, Morse HC, Carbone FR (1992) Murine AIDS superantigen reactivity of the T cells bearing Vβ5 T cell antigen receptor. J Immunol 149:9-16.
Holmes KL, Morse HC, Makino M, Hardy RR, Hayakawa K (1990) A unique subset of normal murine CD4+ T cells lacking Thy‐l is expanded in a murine retrovirus‐induced immunodeficiency syndrome. MAIDS. Eur J Immunol 20:2783-2787.
Thomas PM, Samelson LE (1992) The glycophosphatidylinositolanchored Thy‐l molecule interacts with the p60fyn protein tyrosine kinase in T‐cells. J Biol Chem 267:12317-12322.
Harriman GR, Lycke NY, Elwood LJ, Strober W (1990) Tlymphocytes that express CD4 and the αβ‐T cell receptor but lack Thy‐l. Preferential localization in Peyer's patches. J Immunol 145:2006-2014.
Wadsworth SK, Yui K, Green I (1989) Major histocompalibility complex class 1 specific cytolytic T cells derived form gld mice, lacking Thy‐1. CD4 and CD8. Proc Natl Acad Sci USA 36:1018-1022.
Ohteki T, Seki S, Abo T, Kumagai K (1990) Liver is possible site for the proliferation of abnormal CD3+ 4− 8− double‐negative lymphocytes in autoimmune MRL‐lpr/lpr mice. J Exp Med 172:7-12.
Via CS, Morse HC, Shearer GM (1990) Altered immunoregulation and autoimmune aspects of HIV infection: relevant murine models. Immunol Today 11:250-255.
Budd RC, Cerottini J‐C, Horvath C, Bron C, Pedrazzini T, Howe RC, MacDonald HR (1987) Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp‐1 glycoprotein concomitant with antigenic stimulation. J Immunol 138:3120-3129.
Watanabe H, Ohtsuka K, Kimura M, Ikarashi Y, Ohmori K, Kusumi A, Ohteki T, Seki S, Abo T (1992) Details of an isolation method for hepatic lymphocytes in mice. J Immunol Meth 146:145-154.
Hardy RR (1986) Purilication and coupling of fluorescent proteins for use in flow cytometry. Handbook of Experimental Immunology 1986, Vol 1, Weir DM, Herzenberg LA, Blackwell CC, 4th edn., Oxford:, Blackwell Scientific Publications, Ltd., 31–31; .
Vindelov LL (1977) Flow microfiuorimetric analysis of nuclear DNA in cells from solid tumors and cell suspensions. Virchows Arch (Cell Pathol) 24:227-245.
Hodgson GS, Bradley TR, Marlin RF, Sumner S, Fry P (1975) Recovery of proliferating haematopoietic progenitor cells after killing by hydroxyurea. Cell tissue Kinet 8:51-60.
Rocha B, Freitas AA, Coutinho AA (1983) Population dynamics of T lymphocytes. Renewal rate and expansion in the peripheral lymphoid organs. J Immunol 131:2158-2164.
Abo T, Ohteki T, Seki S, Koyamada N, Yoshikai Y, Masuda T, Rikiishi H, Kumagai K (1991) The appearance of T cell receptor in the liversof mice injected with bacteria. J Exp Med 174:417-424.
Hiromatsu K, Matsuzaki G, Tauchi Y, Yoshikai Y, Nomoto K (1992) Sequential analysis of T cells in the liver during murine listerial infection. J Immunol 149:568-573.
Carlsson SR (1985) Changes in glycan branching and sittlylation of the Thy‐1 antigen during normal differentiation of mouse T‐lymphocytes. Biochem J 226:519-525.
Ermak TH, Steger HJ, Wofsy D (1989) Treatment of murine lupus with monoclonal antibody to L3T4. II. Elfects on immunohistology of thymus. spleen and lymph node. Lab Invest 61:447-456.
Kanagawa O, Wiebenga ME, Vaupel BA (1993) Defective T cell receptor‐mediated signaling and differential induction of T cell functions by murine AIDS virus superantigen. J Immunol 150:1865-1872.
Schanberg LE, Fieenor DE, Kurtzberg J, Haynes BF, Kaufman RE (1991) Isolation and characterization of the genomic human CD7 gene: structural similarity with the murine Thy‐1 gene. Proc Natl Acad Sci USA 88:603-607.
Lcgac E, Autran B, Merle‐Beral H, Katlama C, Debre P (1992) CD4+ CD7− CD57+ T cells: a new T‐lymphocyte subset expanded during human immunodeficiency virus infection. Blood 79:1746-1753.
