High levels of p105 (NFKB1) and p100 (NFKB2) proteins in HPV16-transformed keratinocytes: role of E6 and E7 oncoproteins.

Havard, Laurence; Rahmouni, Souad; Boniver, Jacques; Delvenne, Philippe

doi:10.1016/j.virol.2004.10.030

Article (Scientific journals)

High levels of p105 (NFKB1) and p100 (NFKB2) proteins in HPV16-transformed keratinocytes: role of E6 and E7 oncoproteins.

Havard, Laurence; Rahmouni, Souad; Boniver, Jacques et al.

2005 • In Virology, 331 (2), p. 357-66

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https://hdl.handle.net/2268/4879

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10.1016/j.virol.2004.10.030

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15629778

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Keywords :

Blotting, Western; Cell Line, Transformed; Cell Transformation, Viral/genetics; Gene Expression Regulation, Viral; Humans; Keratinocytes/metabolism/virology; NF-kappa B/metabolism; NF-kappa B p50 Subunit; NF-kappa B p52 Subunit; Oncogene Proteins, Viral/genetics/metabolism; Oncogenes; Papillomaviridae/genetics/metabolism; Repressor Proteins/metabolism; Transcription Factors/metabolism

Abstract :

[en] We have previously shown that functional components of the NF-kappaB signaling pathway are up-regulated and sequestered in the cytoplasm of human papillomavirus 16 (HPV16)-transformed cell lines leading to a reduced activity of NF-kappaB. In this study, we examined the expression of the NF-kappaB precursors p100 and p105 in keratinocytes transformed or not by HPV16. Western immunoblotting experiments demonstrated high levels of p100 and p105 proteins not only in HPV16+ cervical carcinoma-derived keratinocytes but also in keratinocytes stably transfected by HPV16 E6 or E7 oncogenes. Moreover, p100 and p105 proteins were predominantly cytoplasmic and nuclear in keratinocytes expressing E7 and E6, respectively. A predominantly cytoplasmic localization of E7 protein was also detected in all keratinocytes expressing E7. Our results suggest that HPV16 E6 and E7 proteins modulate the expression and the subcellular localization of p100 and p105 NF-kappaB precursors.

Disciplines :

Biochemistry, biophysics & molecular biology
Oncology

Author, co-author :

Havard, Laurence

Rahmouni, Souad ; Université de Liège - ULiège > Département des sciences cliniques > Immunopathologie - Transplantation

Boniver, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique

Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Language :

English

Title :

High levels of p105 (NFKB1) and p100 (NFKB2) proteins in HPV16-transformed keratinocytes: role of E6 and E7 oncoproteins.

Publication date :

2005

Journal title :

Virology

ISSN :

0042-6822

eISSN :

1096-0341

Publisher :

Academic Press, San Diego, United States - California

Volume :

331

Issue :

Pages :

357-66

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 January 2009

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