Synthesis of copolymer brushes endowed with adhesion to stainless steel surfaces and antibacterial properties by controlled nitroxide-mediated radical polymerization
[en] Novel copolymer brushes have been synthesized by a two-step "grafting from" method that consists of the electrografting of poly(2-phenyl-2-(2,2,6,6-tetramethyl-piperidin-1-yloxy)-ethylacrylate) onto stainless steel, followed by the nitroxide-mediated radical polymerization of 2-(dimethylamino ethyl)acrylate and styrene or n-butyl acrylate, initiated from the electrografted polyacrylate chains. The grafted copolymers were quaternized in order to endow them with antibacterial properties. Peeling tests have confirmed the strong adhesion of the grafted copolymer onto the stainless steel substrate. Quartz crystal microbalance experiments have proven that fibrinogen adhesion is lower on the hydrophilic quaternized films compared to the nonionic counterpart. Such quaternized copolymers exhibit significant antibacterial activity against the Gram-positive bacteria S. aureus and the Gram-negative bacteria E. coli.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Ignatova, Miléna; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Voccia, Samuel; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Gilbert, Bernard ; Université de Liège - ULiège > Department of Chemistry > Laboratory of Analytical Chemistry and Electrochemistry
Markova, Nadya; Bulgarian Academy of Sciences, Sofia, Bulgaria > Institute of Microbiology
Mercuri, Paola ; Université de Liège - ULiège > Center for Protein Engineering
Galleni, Moreno ; Université de Liège - ULiège > Center for Protein Engineering
Sciannamea, Valérie; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Lenoir, Sandrine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Cossement, Damien; University of Mons-Hainaut (UMH) > Service de Chimie Inorganique et Analytique (LASSIE)
Gouttebaron, Rachel; University of Mons-Hainaut (UMH) > Service de Chimie Inorganique et Analytique (LASSIE)
Jérôme, Robert ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Language :
English
Title :
Synthesis of copolymer brushes endowed with adhesion to stainless steel surfaces and antibacterial properties by controlled nitroxide-mediated radical polymerization
Publication date :
23 November 2004
Journal title :
Langmuir
ISSN :
0743-7463
eISSN :
1520-5827
Publisher :
Amer Chemical Soc, Washington, United States - Washington
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
Gristina, A. G.; Naylor, P. T. Implant Associated Infection. In Biomaterials Science: An Introduction to Materials in Medicine; Ratner, B. D., Huffman, AS., Schoen, F. J., Lemons, J. E., Eds.; Academic Press: San Diego, CA, 1996; p 205.
An, Y. H.; Friedman, R. J. J. Biomed. Mater. Res. 1998, 43, 338.
Sofia, S. J.; Merrill, E. W. Protein adsorption on poly(ethylene glycol)-grafted silicon surfaces. In Poly(Ethylene Glycol): Chemistry and Biological Applications; Harris, J. M., Zalipsky, S., Eds.; American Chemical Society: Washington, DC, 1997; Chapter 22.
Gombotz, W. R.; Guanghui, W.; Horbett, T. A.; Hoffman, A. S. Protein adsorption to and elution from polyether surfaces. In Poly-(Ethylene Glycol) Chemistry Biotechnical and Biomedical Applications; Harris, J. M., Ed.; Plenum Press: New York, 1992; p 127.
Zhang, M.; Desai, T.; Ferrari, M. Biomaterials 1998, 19, 953.
McPherson, T.; Kidane, A.; Szleifer, I.; Park, K. Langmuir 1998, 14, 176.
Mrksich, M.; Whitesides, G. M. Using self-assembled monolayers that present oligo(ethylene glycol) groups to control the interactions of proteins with surfaces. In Poly(Ethylene Glycol): Chemistry and Biological Applications; Harris, J. M., Zalipsky, S., Eds.; American Chemical Society: Washington, DC, 1997; Chapter 23.
Mrksich, M.; Chen, C. S.; Xia, Y.; Dyke, L. E.; Ingber, D. E.; Whitesides, G. M. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 10775.
Chapman, R. G.; Ostuni, E.; Liang, M. N.; Meluleni, G.; Kim, E.; Yan, L.; Pier, G.; Warren, H. S.; Whitesides, G. M. Langmuir 2001, 17, 1225.
Kato, K.; Sano, S.; Ikada, Y. Colloids Surf., B 1995, 4, 221.
Anders, C.; Gartner, R.; Steinert, V.; Voit, B.; Zschoche, S. J. Macromol. Sci., Chem. 1999, 36 (7/8), 1017.
Rad, A. Y.; Ayhan, H.; Piskin, E. J. Biomed. Mater. Res. 1998, 41, 349.
Isquith, A. J.; Abott, E. A.; Waters, P. A. Appl. Microbiol. 1972, 24, 859.
Walters, P. A.; Abbot, E. A.; Isquith, A. J. Appl. Microbiol. 1973, 25, 253.
Franklin, T. J., Snow, G. A., Eds. Biochemistry of Antimicrobial Action, Antiseptics, Antibiotics, and the Cell Membrane; Chapman & Hall, Ltd.: London, 1981; p 58-78.
Tashiro, T. Macromol. Mater. Eng. 2001, 286, 68.
Prucker, O.; Ruhe, J. Macromolecules 1998, 31, 592.
Tsubokawa, N.; Satoh, M. J. Appl. Polym. Sci. 1997, 65, 2165.
Matyjaszewski, K.; Miller, P. J.; Shukla, N.; Immaraporn, B.; Gelman, A.; Luokala, B. B.; Silclovan, T. M.; Kickelbick, G.; Vallan, T.; Huffman, M.; Pakula, T. Macromolecules 1999, 32, 8716.
Baum, M.; Brittain, W. J. Macromolecules 2002, 35, 610.
Husseman, M.; Malmstrom, E. E.; McNamara, M.; Mate, M.; Mecerreyes, D.; Benoit, D. G.; Hedrick, J. L.; Mansky, P.; Huang, E.; Russel, T. P.; Hawker, C. J. Macromolecules 1999, 32, 1424.
Moller, M.; Nederberg, F.; Lim, L. S.; Kange, R.; Hawker, C. J.; Hedrick, J. L.; Gu, Y.; Shah, R.; Abbott, N. L. J. Polym. Sci., Part A: Polym. Chem. 2001, 39, 3529.
Tsujii, Y.; Ejaz, M.; Sato, K.; Goto, A.; Fukuda, T. Macromolecules 2001, 34, 8872.
Delamar, M.; Désarmot, G.; Fagebaume, O.; Hitmi, R.; Pitson, J.; Savéant, J. M. Carbon 1997, 35, 801.
Yuan, W.; Iroh, J. O. Trends Polym. Sci. 1993, 1, 388.
Claes, M.; Voccia, S.; Detrembleur, C.; Jérôme, C.; Gilbert, B.; Leclère, Ph.; Geskin, V. M.; Gouttebaron, R.; Hecq, M.; Lazzaroni, R.; Jérôme, R. Macromolecules 2003, 36, 5926.
Benoit, D.; Chaplinski, V.; Braslau, B.; Hawker, C. J. J. Am. Chem. Soc. 1999, 121, 3904.
Tanaka, M.; Mochizuki, A.; Shiroya, T.; Motomura, T.; Shimura, K.; Onishi, M.; Okahata, Y. Colloids Surf., A 2002, 203, 195.
Buttry, D. A.; Ward, M. D. Chem. Rev. 1992, 92, 1355.
Franklin, T. J.; Snow, G. A. Biochemistry of Antimicrobial Action; Chapman & Hall: London, 1981; p 58.
Kanazawa, A.; Ikeda, T.; Endo, T. J. Polym. Sci., Part A: Polym. Chem. 1993, 31, 3031.
Mertens, M.; Calberg, C.; Baute, N.; Jérôme, R.; Martinot, L. J. Electroanal. Chem. 1998, 441, 237.
Denes, F.; Manolache, S. Immobilization of Active Biopolymers from Cold Plasma-Functionalized Surfaces for the Creation of Molecular Recognition and Molecular Manufacturing Systems. In Polymeric Biomaterials, 2nd ed. (revised and expanded); Dumitriu, S., Ed.; Marcel Dekker: New York, 2002; p 239-290.
Costerton, J. W.; Cheng, K.-J. J. Antimicrob. Chemother. 1975, 1, 363.
Hugo, W. B.; Russell, A. D. Pharmaceutical Microbiology; Blaokwell (Oxford): Oxford, U.K., 1980; p 3.
