Amino Acid Sequence of the Penicillin-Binding Protein/DD-Peptidase of Streptomyces K15. Predicted Secondary Structures of the Low Mr Penicillin-Binding Proteins of Class A
[en] The low-Mr penicillin-binding protein (PBP)/DD-transpeptidase of Streptomyces K15 is synthesized in the form of a 291-amino acid-residue precursor possessing a cleavable 29-amino acid-residue signal peptide. Sequence-similarity searches and hydrophobic-cluster analysis show that the Streptomyces K15 enzyme, the Escherichia coli PBPs/DD-carboxy-peptidases 5 and 6, the Bacillus subtilis PBP/DD-carboxypeptidase 5 and the spoIIA product (a putative PBP involved in the sporulation of B. subtilis) are structurally related and form a distinct class A of low-Mr PBPs/DD-peptidases. The distribution of the hydrophobic clusters along the amino acid sequences also shows that the Streptomyces K15 PBP, and by extension the other PBPs of class A, have similarity in the polypeptide folding, with the beta-lactamases of class A, with as reference the Streptomyces albus G and Staphylococcus aureus beta-lactamases of known three-dimensional structure. This comparison allows one to predict most of the secondary structures in the PBPs and the amino acid motifs that define the enzyme active sites.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Palomeque-Messia, Pilar; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Englebert, Serge; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Leyh-Bouille, Melina; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Houba, Simone; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Dideberg, Otto; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Van Beeumen, Jozef; Rijksuniversiteit Gent - RUG > Lab voor Microbiologie en microbiële genetica
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Amino Acid Sequence of the Penicillin-Binding Protein/DD-Peptidase of Streptomyces K15. Predicted Secondary Structures of the Low Mr Penicillin-Binding Proteins of Class A