Article (Scientific journals)
Development and validation of a high performance liquid chromatographic method for quantitative determination of aporphine alkaloids from different samples of Cassytha filiformis
Stevigny, C.; Wautier, M. C.; Jiwan, J. L. H. et al.
2004In Planta Medica, 70 (8), p. 764-770
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Keywords :
aporphine alkaloids; Cassytha filiformis; Lauraceae; validation; extraction; quantification; HPLC
Abstract :
[en] A sensitive and accurate procedure based on an alkaloid extraction coupled to an HPLC-UV-MS determination has been developed for the separation and quantification of the major aporphines in Cassytha filiformis. The extraction step and the liquid chromatography conditions were optimized in order to improve the selectivity of the method. The HPLC mobile phase consisted of a mixture of water containing 10 mM ammonium acetate adjusted to pH 3 with acetic acid-acetonitrile (90: 10, v/v) (A) and acetonitrile (B) used in a gradient mode (0 to 40%). The stationary phase was an RP-select B (5 mum) column. The method was completely validated using cassythine, one of the major aporphines in our samples, as reference standard and successfully applied to the determination of these pharmacologically interesting aporphines in seven different batches of C. filiformis. The detection and quantitation limits of cassythine were found to be 13 and 20 mug/mL, respectively. The results showed variations in the total alkaloid content in samples from 0.11 to 0.43%.
Disciplines :
Pharmacy, pharmacology & toxicology
Chemistry
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Stevigny, C.
Wautier, M. C.
Jiwan, J. L. H.
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Quetin-Leclercq, J.
Language :
English
Title :
Development and validation of a high performance liquid chromatographic method for quantitative determination of aporphine alkaloids from different samples of Cassytha filiformis
Publication date :
August 2004
Journal title :
Planta Medica
ISSN :
0032-0943
eISSN :
1439-0221
Publisher :
Georg Thieme Verlag Kg, Stuttgart, Germany
Volume :
70
Issue :
8
Pages :
764-770
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 May 2010

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