[en] BACKGROUND: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions. METHODS: Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 microg bPL/mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers (dilutions ranging from 1:2,500 till 1:1,280,000). RESULT(S): Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower (P < 0.05) in samples added with dilutions of rabbit antiserum lower than 1:80,000 (one foetus) or 1:10,000 (four foetuses). It was also shown that the recovery of added bPL (12 ng/mL) was markedly reduced in those samples in which exogenous rabbit anti-bPL were added at dilutions lower than 1:20,000. Concentrations of foetal bPL were determined in samples from cannulated foetuses. In foetuses 1 and 6, bPL concentrations remained almost unchanged (<5 ng/mL) during the whole experimental period. In Foetus 3, bPL concentrations decreased immediately after IgG infusion and thereafter, they increased until parturition. CONCLUSION(S): The use of a bPL RIA using a guinea pig anti-bPL as primary antiserum allowed for the measurement of bPL concentrations in foetal plasma in presence of rabbit anti-bPL IgG into the foetal circulation. Long-term foetal catheterization allowed for the study of the influence of direct infusion of anti-bPL IgG on peripheral bPL concentrations in bovine foetuses.
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Hornick, Jean-Luc ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Touati, Kamal ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP)
van der Weijden, Gysbert C; University of Utrecht, the Netherlands > Department of Farm Animal Health, Faculty of Veterinary Medicine
Taverne, Marcel Am; University of Utrecht, the Netherlands > Department of Farm Animal Health, Faculty of Veterinary Medicine
Szenci, Otto; Szent Istvan University, Budapest, Hungary > Clinic for Large Animals, Faculty of Veterinary Science
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples.
Goffin, V., Shiverick, K.T., Kelly, P.A., Martial, J.A., Sequence-function relationships within the expanding family of prolactin, growth hormone, placental lactogen, and related proteins in mammals (1996) Endocr Rev, 17, pp. 385-410
Vashdi, D., Elberg, G., Sakal, E., Gertler, A., Biological activity of bovine placental lactogen in 3T3-F442A preadipocytes is mediated through a somatogenic receptor (1992) FEBS Lett, 305, pp. 101-104. , 10.1016/0014-5793(92)80873-F, 1618336
Breier, B.H., Funk, B., Surus, A., Ambler, G.R., Wells, C.A., Waters, M.J., Gluckman, P.D., Characterization of ovine growth hormone (oGH) and ovine placental lactogen (oPL) binding to fetal and adult hepatic tissue in sheep: evidence that oGH and oPL interact with a common receptor (1994) Endocrinology, 135, pp. 919-928. , 10.1210/en.135.3.919, 8070387
Evain-Brion, D., Hormonal regulation of fetal growth (1994) Horm Res, 42, pp. 207-214. , 10.1159/000184195, 7868075
Oliver, M.H., Harding, J.E., Breier, B.H., Evans, P.C., Gallaher, B.W., Gluckman, P.D., The effects of ovine placental lactogen infusion on metabolites, insulin-like growth factors and binding proteins in the fetal sheep (1995) J Endocrinol, 144, pp. 333-338. , 10.1677/joe.0.1440333, 7535836
Currie, M.J., Bassett, N.S., Breier, B.H., Klempt, M., Min, S.H., Mackenzie, D.D., McCutcheon, S.N., Gluckman, P.D., Differential effects of maternal ovine placental lactogen and growth hormone (GH) administration on GH receptor, insulin-like growth factor (IGF)-1 and IGF binding protein-3 gene expression in the pregnant and fetal sheep (1996) Growth Regul, 6, pp. 123-129
Schoknecht, P.A., McGuire, M.A., Cohick, W.S., Currie, W.B., Bell, A.W., Effect of chronic infusion of placental lactogen on ovine fetal growth in late gestation (1996) Domest Anim Endocrinol, 13, pp. 519-528. , 10.1016/S0739-7240(96)00090-2, 8960408
Waters, M.J., Oddy, V.H., McCloghry, C.E., Gluckman, P.D., Duplock, R., Owens, P.C., Brinsmead, M.W., An examination of the proposed roles of placental lactogen in the ewe by means of antibody neutralization (1985) J Endocrinol, 106, pp. 377-386. , 10.1677/joe.0.1060377, 4045344
Shulkes, A., Moore, C., Kolivas, S., Whitley, J., Active immunoneutralization of somatostatin in the sheep: effects on gastrointestinal somatostatin expression, storage and secretion (1999) Regul Pept, 82, pp. 59-64. , 10.1016/S0167-0115(99)00040-3, 10458647
Jones, A.M., Honour, J.W., Unusual results from immunoassays and the role of the clinical endocrinologist (2006) Clin Endocrinol (Oxf), 64, pp. 234-244. , 10.1111/j.1365-2265.2006.02439.x, 16487430
Emerson, J.F., Ngo, G., Emerson, S.S., Screening for interference in immunoassays (2003) Clin Chem, 49, pp. 1163-1169. , 10.1373/49.7.1163, 12816914
Ismail, A.A., Walker, P.L., Fahie-Wilson, M.N., Jassam, N., Barth, J.H., Prolactin and macroprolactin: a case report of hyperprolactinaemia highlighting the interpretation of discrepant results (2003) Ann Clin Biochem, 40, pp. 298-300. , 10.1258/000456303321610673, 12803849
Ward, G., McKinnon, L., Badrick, T., Hickman, P.E., Heterophilic antibodies remain a problem for the immunoassay laboratory (1997) Am J Clin Pathol, 108, pp. 417-421
Piechaczyk, M., Baldet, L., Pau, B., Bastide, J.M., Novel immunoradiometric assay of thyroglobulin in serum with use of monoclonal antibodies selected for lack of cross-reactivity with autoantibodies (1989) Clin Chem, 35, pp. 422-424
Touati, K., Sousa, N.M., Gangl, M., Alvarez-Oxiley, A.V., Revy, D., Weijden, G.C., Taverne, M.A., Beckers, J.F., Investigation on prenatal endocrinology: preliminary results on long term catheterization of bovine foetuses (2004) Reproduction, 31, p. 26. , Abstract serie
Beckers, J.F., De Coster, R., Wouters-Ballman, P., Fromont-Liénard, C., Zwalmen, P., Ectors, F., Dosage radioimmunologique de l'hormone placentaire sommatrope et mammotrope bovine (1982) Annales de Médecine Vétérinaire, 126, pp. 9-21
Gootwine, E., Placental hormones and fetal-placental development (2004) Anim Reprod Sci, 82-83, pp. 551-566. , 10.1016/j.anireprosci.2004.04.008, 15271479
Wooding, F.B., Beckers, J.F., Trinucleate cells and the ultrastructural localisation of bovine placental lactogen (1987) Cell Tissue Res, 247, pp. 667-673. , 10.1007/BF00215761, 3568109
Beckers, J.F., Fromont-Liénard, C., Zwalmen, P., Wouters-Ballman, P., Ectors, F., Isolement d'une hormone placentaire bovine présentant une activité analogue à la prolactine et à l'hormone de croissance (1980) Annales de Médecine Vétérinaire, 124, pp. 585-601
Vaitukaitis, J., Robbins, J.B., Nieschlag, E., Ross, G.T., A method for producing specific antisera with small doses of immunogen (1971) J Clin Endocrinol Metab, 33, pp. 988-991. , 10.1210/jcem-33-6-988, 5316354
Alvarez-Oxiley, A.V., Sousa, N.M., Hornick, J.L., Touati, K., Weijden, G.C., Taverne, M.A., Szenci, O., Beckers, J.F., Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation (2007) Reprod Fertil Dev, 19, pp. 877-885. , 10.1071/RD06173, 17897591
Harboe, N., Ingild, A., Immunization, isolation of immunoglobulins, estimation of antibody titre (1973) Scand J Immunol Suppl, 1, pp. 161-164. , 10.1111/j.1365-3083.1973.tb03798.x, 4204108
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
Rexroad, C.E., Casida, L.E., Tyler, W.J., Crown-rump length of fetuses in purebred Holstein-Friesan cows (1974) J Dairy Sci, 57, pp. 346-347
Taverne, M.A., Bevers, N.M., Weyden, G., Dieleman, S.J., Fontijne, P., Concentration of growth hormone, prolactin and cortisol in fetal and maternal blood and amniotic fluid during late pregnancy and parturition in cows with cannulated fetuses (1988) Anim Reprod Sci, 17, pp. 51-59
Statistical Analysis System, Statistic Institute Inc., Version 5.0, (1998) Cary NC, USA, , SAS User's Guide
Culler, M.D., Negro-Vilar, A., Passive immunoneutralization: a method for studying the regulation of basal and pulsatile hormone secretion (1989) Methods Enzymol, 168, pp. 498-516. , full_text, 2566893
Handwerger, S., Clinical counterpoint: the physiology of placental lactogen in human pregnancy (1991) Endocr Rev, 12, pp. 329-336. , 10.1210/edrv-12-4-329, 1662129
Anthony, R.V., Pratt, S.L., Liang, R., Holland, M.D., Placental-fetal hormonal interactions: impact on fetal growth (1995) J Anim Sci, 73, pp. 1861-1871
Eakle, K.A., Arima, Y., Swanson, P., Grimek, H., Bremel, R.D., A 32,000-molecular weight protein from bovine placenta with placental lactogen-like activity in radioreceptor assays (1982) Endocrinology, 110, pp. 1758-1765. , 10.1210/endo-110-5-1758, 7075536
Murthy, G.S., Schellenberg, C., Friesen, H.G., Purification and characterization of bovine placental lactogen (1982) Endocrinology, 111, pp. 2117-2124. , 10.1210/endo-111-6-2117, 7140648
Arima, Y., Bremel, R.D., Purification and characterization of bovine placental lactogen (1983) Endocrinology, 113, pp. 2186-2194. , 10.1210/endo-113-6-2186, 6641631
Byatt, J.C., Shimomura, K., Duello, T.M., Bremel, R.D., Isolation and characterization of multiple forms of bovine placental lactogen from secretory granules of the fetal cotyledon (1986) Endocrinology, 119, pp. 1343-1350. , 10.1210/endo-119-3-1343, 3732169
Byatt, J.C., Wallace, C.R., Bremel, R.D., Collier, R.J., Bolt, D.J., The concentration of bovine placental lactogen and the incidence of different forms in fetal cotyledons and in fetal serum (1987) Domest Anim Endocrinol, 4, pp. 231-241. , 10.1016/0739-7240(87)90019-1, 3507892
Mariotti, S., Barbesino, G., Caturegli, P., Marino, M., Manetti, L., Pacini, F., Centoni, R., Pinchera, A., Assay of thyroglobulin in serum with thyroglobulin autoantibodies: an unobtainable goal? (1995) J Clin Endocrinol Metab, 80, pp. 468-472. , 10.1210/jc.80.2.468, 7852506
Spencer, C.A., Takeuchi, M., Kazarosyan, M., Wang, C.C., Guttler, R.B., Singer, P.A., Fatemi, S., Nicoloff, J.T., Serum thyroglobulin autoantibodies: prevalence, influence on serum thyroglobulin measurement, and prognostic significance in patients with differentiated thyroid carcinoma (1998) J Clin Endocrinol Metab, 83, pp. 1121-1127. , 10.1210/jc.83.4.1121, 9543128
Armin, J., Cunningham, N.F., Grant, R.T., Lloyd, M.K., Wright, P.H., Acute insulin deficiency provoked in the dog, pig and sheep by single injections of anti-insulin serum (1961) J Physiol, 157, pp. 64-73. , 1359916, 16992079
Sapin, R., The interference of insulin antibodies in insulin immunometric assays (2002) Clin Chem Lab Med, 40, pp. 705-708. , 10.1515/CCLM.2002.121, 12241018
Leibovich, H., Gertler, A., Bazer, F.W., Gootwine, E., Active immunization of ewes against ovine placental lactogen increases birth weight of lambs and milk production with no adverse effect on conception rate (2000) Anim Reprod Sci, 64, pp. 33-47. , 10.1016/S0378-4320(00)00198-6, 11078965
Schneider, A.B., Pervos, R., Radioimmunoassay of human thyroglobulin: effect of antithyroglobulin autoantibodies (1978) J Clin Endocrinol Metab, 47, pp. 126-137. , 10.1210/jcem-47-1-126, 263287
Holland, M.D., Hossner, K.L., Williams, S.E., Wallace, C.R., Niswender, G.D., Odde, K.G., Serum concentrations of insulin-like growth factors and placental lactogen during gestation in cattle. I. Fetal profiles (1997) Domest Anim Endocrinol, 14, pp. 231-239. , 10.1016/S0739-7240(97)00023-4, 9260061
Comline, R.S., Hall, L.W., Lavelle, R.B., Nathanielsz, P.W., Silver, M., Parturition in the cow: endocrine changes in animals with chronically implanted catheters in the foetal and maternal circulations (1974) J Endocrinol, 63, pp. 451-472. , 10.1677/joe.0.0630451, 4375697
Bauer, M.K., Harding, J.E., Breier, B.H., Gluckman, P.D., Exogenous GH infusion to late-gestational fetal sheep does not alter fetal growth and metabolism (2000) J Endocrinol, 166, pp. 591-597. , 10.1677/joe.0.1660591, 10974653
Taylor, M.J., Jenkin, G., Robinson, J.S., Thorburn, G.D., Friesen, H., Chan, J.S., Concentrations of placental lactogen in chronically catheterized ewes and fetuses in late pregnancy (1980) J Endocrinol, 85, pp. 27-34. , 10.1677/joe.0.0850027, 7391722
Reynolds, L.P., Ferrell, C.L., Robertson, D.A., Klindt, J., Growth hormone, insulin and glucose concentrations in bovine fetal and maternal plasmas at several stages of gestation (1990) J Anim Sci, 68, pp. 725-733
Sangild, P.T., Schmidt, M., Jacobsen, H., Fowden, A.L., Forhead, A., Avery, B., Greve, T., Blood chemistry, nutrient metabolism, and organ weights in fetal and newborn calves derived from in vitro-produced bovine embryos (2000) Biol Reprod, 62, pp. 1495-1504. , 10.1095/biolreprod62.6.1495, 10819749
Cunningham, N.F., Patterson, D.S., Wright, P.H., Acute insulin deficiency provoked in sheep and cows by single injections of anti-insulin serum (1963) J Physiol, 169, pp. 137-148. , 1368706, 14078054
Holst, J.J., Galbo, H., Richter, E.A., Neutralization of glucagon by antiserum as a tool in glucagon physiology. Lack of depression of basal blood glucose after antiserum treatment in rats (1978) J Clin Invest, 62, pp. 182-190. , 10.1172/JCI109104, 371752, 659632
Almdal, T.P., Holst, J.J., Heindorff, H., Vilstrup, H., Glucagon immunoneutralization in diabetic rats normalizes urea synthesis and decreases nitrogen wasting (1992) Diabetes, 41, pp. 12-16. , 10.2337/diabetes.41.1.12, 1727732
Kaneko, H., Todoroki, J., Noguchi, J., Kikuchi, K., Mizoshita, K., Kubota, C., Yamakuchi, H., Perturbation of estradiol-feedback control of luteinizing hormone secretion by immunoneutralization induces development of follicular cysts in cattle (2002) Biol Reprod, 67, pp. 1840-1845. , 10.1095/biolreprod.102.007591, 12444061
Kerr, D.E., Laarveld, B., Manns, J.G., Effects of passive immunization of growing guinea-pigs with an insulin-like growth factor-I monoclonal antibody (1990) J Endocrinol, 124, pp. 403-415. , 10.1677/joe.0.1240403, 2159044
Spencer, G.S., Hodgkinson, S.C., Bass, J.J., Passive immunization against insulin-like growth factor-I does not inhibit growth hormone-stimulated growth of dwarf rats. Endocrinology (1991), 128, pp. 2103-2109
Handwerger, S., Maurer, W.F., Crenshaw, C., Hurley, T., Barrett, J., Fellows, R.E., Development of the sheep as an animal model to study placental lactogen physiology (1975) J Pediatr, 87, pp. 1139-1143. , 10.1016/S0022-3476(75)80128-4, 1185414
Martal, J., Djiane, J., The production of chorionic somatomammotrophin in sheep (1977) J Reprod Fertil, 49, pp. 285-289
Butler, W.R., Fullenkamp, S.M., Cappiello, L.A., Handwerger, S., The relationship between breed and litter size in sheep and maternal serum concentrations of placental lactogen, estradiol and progesterone (1981) J Anim Sci, 53, pp. 1077-1081
Bomford, R., Aston, R., Enhancement of bovine growth hormone activity by antibodies against growth hormone peptides (1990) J Endocrinol, 125, pp. 31-38. , 10.1677/joe.0.1250031, 2338531
Massart, S., Maiter, D., Portetelle, D., Adam, E., Renaville, R., Ketelslegers, J.M., Monoclonal antibodies to bovine growth hormone potentiate hormonal activity in vivo by enhancing growth hormone binding to hepatic somatogenic receptors (1993) J Endocrinol, 139, pp. 383-393. , 10.1677/joe.0.1390383, 8133207
Wang, B.S., Search, D.J., Lumanglas, A.A., Ingling, J., Corbett, M.J., Shieh, H.M., Kraft, L.A., Augmentation of hormonal activities with antibodies from cattle immunized with a combination of synthetic and recombinant growth hormone peptide (1998) Anim Biotechnol, 9, pp. 121-133. , 10.1080/10495399809525899, 9713677
Conley, L.K., Gaillard, R.C., Giustina, A., Brogan, R.S., Wehrenberg, W.B., Effects of repeated doses and continuous infusions of the growth hormone-releasing peptide hexarelin in conscious male rats (1998) J Endocrinol, 158, pp. 367-375. , 10.1677/joe.0.1580367, 9846166
