Set-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
[en] The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic
and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge.
In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the
adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa,
lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation.
Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl.
The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and
differentiation.
Set-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
