Article (Scientific journals)
Electrical microenvironment influence on the hydrolytic activity of free and immobilised Yarrowia lipolytica lipase
Paquot, Michel; Deleu, Magali; Beaufils, C. et al.
1996In Biotechnology Letters, 18 (1), p. 73-78
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Abstract :
[en] The role of electrical properties of interfaces upon the activity of free and immobilised Yarrowia lipolytica lipase has been investigated. Sodium taurocholate and Sedipur 400, an anionic polyacrylamide, enhance the negative character of the fatty droplets of substrate and tend to improve the lipolytic activity while the cationic polyacrylamide (Sedipur 900) has opposite effects. Ca+ which reduces the fatty droplets charge as Sedipur 900, is however a good activator of the enzyme. The role of electrical properties on the optimum pH of the immobilised enzyme is clearer. Immobilisation of the lipase on a positively charged support shifts its optimum pH to acidic pH by repulsion towards H+ ions around the support.
Disciplines :
Agriculture & agronomy
Author, co-author :
Paquot, Michel ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Deleu, Magali  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Beaufils, C.
Blecker, Christophe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Language :
English
Title :
Electrical microenvironment influence on the hydrolytic activity of free and immobilised Yarrowia lipolytica lipase
Publication date :
1996
Journal title :
Biotechnology Letters
ISSN :
0141-5492
eISSN :
1573-6776
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
18
Issue :
1
Pages :
73-78
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 March 2010

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