Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal stem cells.

[en] Mesenchymal stem cells (MSCs) are studied as a cellular source for the treatment of various diseases. In this work, we isolated and cultivated murine bone marrow-derived MSCs. After a first observation of a solid tumour in a mouse injected with these cells, we systematically explored their chromosomal stability. We observed in all the cytogenetically analysed cases gross chromosomal alterations every time the MSCs went through the senescence crisis while the lymphocytes from the same animals showed a normal chromosome count. This observation was confirmed in different mouse strains, with different culture protocols, and even in short-term cultures after an hematopoietic cell negative immunodepletion performed in order to accelerate the isolation procedure. Therefore, we conclude that murine MSCs display high chromosomal instability, can generate tumours, and that care must be taken before using them for the evaluation of MSC therapeutic potential.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Josse, Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine

Schoemans, R.

Niessen, Neville-Andrew ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau

Delgaudine, Marie ; Centre Hospitalier Universitaire de Liège - CHU > Radiothérapie

Hellin, Anne-Cécile ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Herens, Christian ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Bours, Vincent ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Language :

English

Title :

Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal stem cells.

Publication date :

August 2010

Journal title :

Stem Cells and Development

ISSN :

1547-3287

eISSN :

1557-8534

Publisher :

Mary Ann Liebert, Inc.

Volume :

Issue :

Pages :

1167-1173

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 February 2010

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Bibliography

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