Reference : Comparative Evaluation of Four Detectors in the High-Performance Liquid Chromatograph...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Comparative Evaluation of Four Detectors in the High-Performance Liquid Chromatographic Analysis of Chiral Nonaromatic Alcohols
Toussaint, B. [> > > >]
Duchateau, A. L. [> > > >]
van der Wal, S. [> > > >]
Albert, Adelin mailto [Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique >]
Hubert, Philippe mailto [Université de Liège - ULiège > > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Journal of Chromatographic Science
Yes (verified by ORBi)
[en] A comparative evaluation of ultraviolet, polarimetric, refractive index, and evaporative light-scattering detection coupled with high-performance liquid chromatography has been developed for the separation and quantitation of the enantiomers of chiral nonaromatic alcohols, some of which are intermediates in the synthesis of chiral drugs. (R,S)-3-tert-butylamino-1,2-propanediol; (R,S)-glycidol; and (R,S)-1-(4-morpholino)-2-octanol are selected as model compounds in order to compare the detection sensitivity and the linearity of the response with the four detectors. Separation of the enantiomers is performed using chiral stationary phases in normal-phase liquid chromatography. A one-day validation is achieved for (S)-3-tert-butylamino-1,2-propanediol with each detector, and limits of quantitation are determined for the three compounds. Advantages and limitations of the four detectors are discussed.

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