Molecular cloning and characterisation of the CD18 partner in ovine (Ovis aries) beta(2)-integrins

[en] The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the ovine beta(2) (CD18) subunit, common to the leukocyte beta(2)-integrin family. The deduced 770-amino-acid sequence reveals a transmembrane protein with 81%, 83% and 95% identity with its murine, human and bovine homologues, respectively. Comparisons of CD18 sequences emphasize the functional importance of the beta(2) subunit I-like domain and included metal ion-dependent adhesion site (MIDAS)-like motif and confirm that of the cytoplasmic tail. The data provided here will offer the possibility to explore new avenues in studies based on the ovine model. (C) 2004 Elsevier B.V. All rights reserved.

Disciplines :

Genetics & genetic processes

Author, co-author :

Zecchinon, Laurent ; Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies

Fett, Thomas ; Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies

Baise, Etienne ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Desmecht, Daniel ; Université de Liège - ULiège > Département de Morphologie et Pathologie > Pathologie spéciale et autopsies

Language :

English

Title :

Molecular cloning and characterisation of the CD18 partner in ovine (Ovis aries) beta(2)-integrins

Publication date :

2004

Journal title :

Gene

ISSN :

0378-1119

eISSN :

1879-0038

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

334

Pages :

47-52

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 January 2010

Statistics

Number of views

47 (4 by ULiège)

Number of downloads

1 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Abraham W., Gill A., Ahmed A., Sielczak M.W., Lauredo I.T., Botinnikova Y., Lin K.C., Pepinsky B., Leone D.R., Lobb R.R., Adams S.P. A small molecule, tight-binding inhibitor of the integrin alpha (4) beta (1) blocks antigen induced responses and inflammation in experimental asthma in sheep. Am. J. Respir. Crit. Care Med. 162:2000;603-611
Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410
Bailly P., Tontti E., Hermand P., Cartron J.P., Gahmberg C.G. The red cell LW blood group protein is an intercellular adhesion molecule which binds to CD11/CD18 leukocyte integrins. Eur. J. Immunol. 25:1995;3316-3320
Bischof R.J., Snibson K., Shaw R., Meeusen E.N. Induction of allergic inflammation in the lungs of sensitized sheep after local challenge with house dust mite. Clin. Exp. Allergy. 33:2003;367-375
Bork P., Doerks T., Springer T., Snel B. Domains in plexins: links to integrins and transcription factors. Trends Biochem. Sci. 24:1999;261-263
Chatila T., Geha R., Arnaout M. Constitutive and stimulus-induced phosphorylation of CD11/CD18 leucocyte adhesion molecules. J. Cell Biol. 109:1989;3435-3444
Collie D.D. Comparative, complementary and relevant: the immunological basis of ovine lung allergic responses. Clin. Exp. Allergy. 33:2003;282-286
Fulka J., First N., Loi P., Moor R. Cloning by somatic cell nuclear transfer. BioEssays. 20:1998;847-851
Gahmberg C. Leukocyte adhesion: CD11/CD18 integrins and intercellular adhesion molecules. Curr. Opin. Cell Biol. 9:1997;643-650
Hibbs M., Xu H., Stacker S., Springer T. Regulation of adhesion to ICAM-1 by the cytoplasmic domain of LFA-1 integrin beta subunit. Science. 251:1991;1611-1613
Huang C., Zang Q., Takagi J., Springer T. Structural and functional studies with antibodies to the integrin β2 subunit. J. Biol. Chem. 275:2000;21514-21524
Hynes R. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 69:1992;11-25
Jensen L.J., Gupta R., Blom N., Devos D., Tamames J., Kesmir C., Nielsen H., Staerfeldt H.H., Rapacki K., Workman C., Andersen C.A., Knudsen S., Krogh A., Valencia A., Brunak S. Prediction of human protein function from post-translational modifications and localization features. J. Mol. Biol. 319:2002;1257-1265
Kishimoto T., O'Connor K., Lee A., Roberts T., Springer T. Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family. Cell. 48:1987;681-686
Mitazaka M., Trnka Z. Sheep as an experimental model of immunology: immunological techniques in vitro and in vivo. Immunol. Methods. 3:1985;403-423
Nicholas, B., Karl, B., Nicholas, P., Hugh Jr., B., 1997. GeneDoc: a tool for editing and annotating multiple sequence alignments, http://www.pscedu/ biomed/genedoc.
Nielsen H., Engelbrecht J., Brunak S., von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1997;1-6
Rozen S., Skaletsky H.J. Primer3 on the WWW for general users and for biologist programmers. (http://www-genome.wi.mit.edu/genome_software/other/ primer3.html) Krawetz S., Misener S. Bioinformatics Methods and Protocols: Methods in Molecular Biology. 2000;365-386 Humana Press, Totowa
Shuster D., Bosworth B., Kehrli M. Sequence of the bovine CD18-encoding cDNA: comparison with the human and murine glycoprotéins. Gene. 114:1992;267-271
Springer T. Adhesion receptors of the immune system. Nature. 346:1990;425-434
Tian L., Yoshihara Y., Mizuno T., Mori K., Gahmberg C.G. The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin. J. Immunol. 158:1997;928-936
Weitzman J., Wells C., Wright A., Clark P., Law A. The gene organization of the human β2 integrin subunit (CD18). FEBS. 294:1991;97-103
Wilson R., O'Brien W., Beaudet A. Nucleotide sequence of the cDNA from the mouse leukocyte adhesion protein CD18. Nucleic Acids Res. 17:1989;5397-5398
Zanjani E., Almeida-Porada G., Ascensao J., MacKintosh F., Flake A. Transplantation of hematopoietic stem cells in utero. Stem Cells. 15(Suppl. 1):1997;79-92