Reference : A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain ...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/35031
A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain rearrangement in cis in mice and in the 103/bcl2 cell line
English
Cocea, L. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
De Smet, A. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
Saghatchian, M. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
Fillatreau, S. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
Ferradini, L. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
Schurmans, Stéphane mailto [Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Institut de Biologie et de Médecine Moléculaire > > >]
Weill, J. C. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
Reynaud, A. [Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker, Paris, France > > > >]
1999
Journal of Experimental Medicine
Rockefeller University Press
189
1443-1450
Yes (verified by ORBi)
International
0022-1007
1540-9538
New York
NY
[en] regulation of rearrangement ; mouse immunoglobulin genes ; allelic exclusion ; germline promoter ; positive regulatory element
[en] We have shown previously that a mutation of the KI-KII site immediately 5' to J(kappa)1 on the mouse immunoglobulin light chain kappa locus reduces the rearrangement level in cis, although it does not affect transcription. Here we deleted by homologous recombination in mouse embryonic stem cells a 4-kb DNA fragment, located immediately upstream of the KI-KII element, which contains the promoter of the long germline transcript. Analysis of gene-targeted heterozygous mouse splenic B cells showed a strong decrease in rearrangement for the allele bearing the deletion. When both the KI-KII mutation and the 4-kb deletion were present on the same allele, the overall reduction in rearrangement was stronger than with the 4-kb deletion alone underlying the role of these two elements in the regulation of rearrangement. The same deletion was performed by homologous recombination on one allele of the rearrangement-inducible mouse 103/bcl2-hygro(R) pre-B cell line, and resulted in a similar reduction in the induction of rearrangement of the mutated allele. This result validates this cell line as an in vitro model for studying the incidence of gene-targeted modifications of the kappa locus on the regulation of rearrangement
Researchers ; Professionals
http://hdl.handle.net/2268/35031
10.1084/jem.189.9.1443
Abbreviations used in this paper: BCR, B cell receptor; ES, embryonic stem; RT, reverse transcription

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