thyroid; protooncogenes; antioncogenes; proliferation; cancer
Abstract :
[en] he aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A first study has allowed to identify three new genes. Further search will use the differential display and gene arrays methodology. The role of the identified proteins coded by the genes is studied in vitro by the search of partner proteins by the double hybrid method and in vivo by mice gene knockout technology
Goffard, J. C.; Université Libre de Bruxelles - ULB
Dumont, J. E.; Université Libre de Bruxelles - ULB
Schurmans, Stéphane ; Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucléaire, Institut de Biologie et de Médecine Moléculaire
MaenhauT, C.; Université Libre de Bruxelles - ULB
Language :
English
Title :
Identification and characterization of mRNAs differentially expressed in thyroid cells stimulated by a mitogenic treatment
Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, NY.
StJohn T.P., Davis R.W. Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisaeby differential plaque filter hybridization. Cell. 16:1979;443-452.
Linzer D., Nathan D. Growth-related changes in specific mRNAs of cultured mouse cells. Proc. Natl. Acad. Sci. USA. 80:1993;4271-4275.
Cochran B., Reffel A., Stoiles C. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 33:1983;939-947.
Sive H.L., St John T.P. A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction. Nucleic Acids Res. 16:1988;10937.
Lanahan A., Williams J.B., Sanders L.K., Nathans D. Growth factor-induced delayed early response genes. Mol. Cell Biol. 12:1992;3919-3929.
Sagerström C., Sun B., Sive H. Subtractive cloning: past, present, and future. Annu. Rev. Biochem. 66:1997;751-783.
Liang P., Pardee A.B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science. 257:1992;967-971.
Lisitsyn N., Lisitsyn N., Wigler M. Cloning the differences between two complex genomes. Science. 259:1993;946-951.
Hubank M., Schatz D.G. Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res. 22:1994;5640-5648.
Velculescu V.E., Zhang L., Vogelstein B., Kinzler K.W. Serial analysis of gene expression. Science. 270:1995;484-487.
Yang M., Sytkowski A.J. Cloning differentially expressed genes by linker capture subtraction. Anal. Biochem. 237:1996;109-114.
Diatchenko L., Lau Y.F., Campbell A.P., Chenchik A., Mogadam F., Huang B., Lukyanov S., Lykanov K., Gurskaya N., Sverdlov E., Siebert P. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA. 93:1996;6025-6030.
Suzuki Y., Sato N., Tohyama M., Wanaka A., Takagi T. Efficient isolation of differentially expressed genes by means of a newly established method, 'ESD'. Nucleic Acids Res. 24:1996;797-799.
Liang P., Averboukh L., Pardee A. Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 21:1993;3269-3275.
Wang X., Feuerstein G.Z. The use of mRNA differential display for discovery of novel therapeutic targets in cardiovascular disease. Cardiovascular Res. 35:1997;414-421.
Okubo K., Hori N., Matoba R., Nilyama T., Fukushima A., Kojima Y., Matsubara K. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nature Genet. 2:1992;173-179.
Adams M.D., Soares M.B., Kerlavage A.R., Fields C., Venter J.C. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nature Genet. 4:1993;373-380.
Roger P.P., Dumont J.E. Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors. Mol. Cell Endocrinol. 36:1984;79-93.
Roger P.P., Van Heuverswyn B., Lambert C., Reuse S., Vassart G., Dumont J.E. Antagonistic effects of thyrotropin and epidermal growth factor on thyroglobulin mRNA level in cultured thyroid cells. Eur. J. Biochem. 152:1985;239-245.
Roger P.P., Reuse S., Servais P., Van Heuverswyn B., Dumont J.E. Stimulation of cell proliferation and inhibition of differentiation expression by tumor-promoting phorbol esters in dog thyroid cells in primary culture. Cancer Res. 46:1986;898-906.
Gérard C., Roger P.P., Dumont J.E. Thyroglobulin gene expression as a differentiation marker in primary cultures of calf thyroid cells. Mol. Cell. Endocrinol. 61:1989;23-35.
Maenhaut C., Brabant G., Vassart G., Dumont J.E. In vitro and in vivo regulation of thyrotropin receptor mRNA levels in dog and human thyroid cells. J. Biol. Chem. 267:1992;3000-3007.
Miot F., Wilkin F., Dremier S., Uyttersprot N., Lamy F., Dumont J.E., Maenhaut C. Cloning of cDNA specifically involved in the thyroid cAMP mitogenic pathway. Hormone Res. 42:1994;27-30.
Wilkin F., Savonet V., Radulescu A., Petermans J., Dumont J.E., Maenhaut C. Identification and characterization of novel genes modulated in the thyroid of dogs treated with methimazole and propylthiouracil. J. Biol. Chem. 271:1996;28451-28457.
Maru Y., Witte O. The BCR gene encodes a novel serine/threonine kinase activity within a single exon. Cell. 67:1991;459-468.
Beeler J.F., Larochette W.J., Chedid M., Tronick S.R., Aaronsen S.A. Prokaryotic expression cloning of a novel human tyrosine kinase. Mol. Cell Biol. 14:1994;982-988.
Eichinger L., Bomblies L., Vandekerckhove J., Schleicher M., Gettemans J. A novel type of protein kinase phosphorylates actin in the actin-fragmin. EMBO J. 15:1996;5547-5556.
Wilkin F., Suarez-Huerta N., Robaye B., Peetermans J., Libert F., Dumont J.E., Maenhaut C. Characterization of a phosphoprotein whose mRNA is regulated by the mitogenic pathways in dog thyroid cells. Eur. J. Biochem. 248:1997;660-668.
Watanabe G., Saito Y., Madaule P., Ishizaki T., Fujisawa K., Morii N., Mukai H., Ono Y., Kakizuka A., Naruniya S. Protein kinase N (PKN) and PKN-related protein rhopilin as targets of small GTPase Rho. Science. 271:1996;645-648.
Wan J., Sharp S., Poirier G., Wagaman P., Chambers J., Pyab J., Hom Y.L., Galindo J.E., Huvar A., Peterson P., Jackson M., Erlander M. Cloning differentially expressed mRNAs. Nature Biotechnol. 14:1996;1685-1691.
Lockhart D., Dong H., Byrne M., Folletie M., Gallo M., Chee M., Mittmann M., Wang C., Kobayashi M., Horton H., Brown E. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nature Biotechnol. 14:1996;1675-1680.
Derisi J., Penland L., Brown P., Bittner M., Meltzer P.S., Ray M., Chen Y., Su Y., Trent J. Use of a cDNA microarray to analyse gene expression patterns in human cancer. Nature Genet. 14:1996;457-460.
Heller R., Schena M., Cahi A., Shalon D., Bedilion T., Gilmore J., Woolley D., Davis R. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc. Natl. Acad. Sci. USA. 94:1997;2150-2155.
Ramsay G. DNA chips: State-of the art. Nature Biotechnol. 16:1998;40-44.
Fields S., Song O.K. A novel genetic system to detect protein-protein interactions. Nature. 340:1989;245-246.
Mansour S.L., Thomas K.R., Capecchi M.R. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-slectable genes. Nature. 336:1988;348-352.
Schurmans S., Schiffmann S.N., Gurden H., Lemaire M., Lipp H.P., Schwam V., Pochet R., Imperato A., Bohme G.A., Parmentier M. Impaired long-term potentiation induction in dentate gyrus of calretinin-deficient mice. Proc. Natl. Acad. Sci. USA. 94:1997;10415-10420.
