Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.

Korka, Vasiliki; Petropoulos, Apostolos; Ioannidou, Sofia Maria; Lin, Carol Sze Ki; Koutinas, Apostolis; Fickers, Patrick

doi:10.1093/femsyr/foaf052

Article (Scientific journals)

Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.

Korka, Vasiliki; Petropoulos, Apostolos; Ioannidou, Sofia Maria et al.

2025 • In FEMS Yeast Research, 25

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https://hdl.handle.net/2268/348991

DOI
10.1093/femsyr/foaf052

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40972046

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Keywords :

biorefineries; life cycle assessment; metabolic engineering; succinic acid; technoeconomic evaluation; yeast; Succinic Acid; Industrial Microbiology/methods; Biosynthetic Pathways; Metabolic Engineering/methods; Succinic Acid/metabolism; Yarrowia/metabolism; Yarrowia/genetics; Yeasts/metabolism; Yeasts/genetics; Industrial Microbiology; Yarrowia; Yeasts; Microbiology; Applied Microbiology and Biotechnology

Abstract :

[en] This review highlights the potential of Yarrowia lipolytica and other yeasts as sustainable producers of bio-based succinic acid (SA), a key platform chemical with applications in bioplastics, solvents, and pharmaceuticals. Recent advances in metabolic engineering have substantially improved SA titers, yields, and productivities in yeasts. These improvements were achieved by reconstructing biosynthetic pathways, disrupting gene involved in side-metabolism and/or expressing heterologous genes involved in critical metabolic functions. The use of renewable feedstocks, including crude glycerol, agricultural residues, food waste hydrolysates, and industrial by-products, has shown promise in reducing both production costs and environmental impacts. Innovative downstream separation techniques, such as in situ extraction, membrane filtration, and crystallization, further contribute to process sustainability. Integrating yeast-based SA production into circular biorefineries and adopting continuous production systems are promising strategies for enhancing economic feasibility and minimizing ecological footprints. Although challenges related to scale-up and process integration persist, ongoing advancements in genetic engineering and bioprocessing technologies position yeast-based processes as a viable route for sustainable, large-scale bio-based SA production within a circular bioeconomy framework.

Precision for document type :

Review article

Disciplines :

Biotechnology

Author, co-author :

Korka, Vasiliki ; Université de Liège - ULiège > TERRA Research Centre > Microbial technologies ; Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

Petropoulos, Apostolos ; Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

Ioannidou, Sofia Maria ; Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

Lin, Carol Sze Ki ; School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China

Koutinas, Apostolis ; Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial technologies

Language :

English

Title :

Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.

Publication date :

16 September 2025

Journal title :

FEMS Yeast Research

ISSN :

1567-1356

eISSN :

1567-1364

Publisher :

Oxford University Press

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://academic.oup.com/femsyr/advance-article-pdf/doi/10.1093/femsyr/foaf052/64285949/foaf052.pdf

Funders :

Onassis Foundation

Funding text :

Vasiliki Korka was supported by the Onassis Foundation Scholarship [Scholarship ID: F ZU 037-1/2024-2025].

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