[en] RNA interference (RNAi) has been used for agricultural insect pest control based on silencing of targeted insect genes. However, the effectiveness of RNAi and its applications in insect pest control remain challenging. Here we review factors that may affect the effectiveness of RNAi application, including the variability in RNAi efficacy among different insect species, a limited understanding of double-stranded RNA (dsRNA) uptake and systemic RNAi mechanisms, and the effective delivery of dsRNA in field conditions. Furthermore, we summarize recent progress in RNAi strategies for crop protection, discuss the advantages and disadvantages of RNAi-based insect control, and propose potential strategies to increase the effectiveness of RNAi in insect control.
Disciplines :
Entomology & pest control
Author, co-author :
Zhong, Xue ✱; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI) ; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs (Hainan)/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China
Yu, Xiudao ✱; Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants, College of Life Sciences, Gannan Normal University, Ganzhou, China
Zhang, Jiahui ✱; College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, China
Xu, Jiajing ; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs (Hainan)/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China
Qin, Mengchao ; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs (Hainan)/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China
Cao, Mingxin; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs (Hainan)/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI)
Xia, Lanqin; State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs (Hainan)/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, China
✱ These authors have contributed equally to this work.
Language :
English
Title :
RNAi technologies for insect control in crop protection
We apologize to those whose work we were unable to cite due to space and reference limitations. Part of work mentioned in this review is funded by the National Natural Science Foundation of China (32188102 to Lanqin Xia) and Natural Science Foundation for Distinguished Young Scholars of Jiangxi province (20212ACB215001 to Xiudao Yu). Xue Zhong was supported by the China Scholarship Council (202303250062) and the GSCAAS-ULg Joint PhD Program.
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