Genome Sequences of Three Enterococcus faecalis Strains (LAB1, LAB10, and LAB11) with Probiotic, Plant Growth-Promoting, and Nitrifying Properties - 2026
[en] Here we report the draft genome sequences of three Enterococcus faecalis strains, LAB1, LAB10, and LAB11, isolated from the pond water of a tilapia (Oreochromis niloticus) aquaculture farm at the University Nangui Abrogoua, Abidjan, Ivory Coast. These strains were previously characterised for their probiotic, plant growth-promoting (PGP), and nitrifying properties. All three strains were assigned to sequence type ST19 by multilocus sequence typing (MLST). The draft genomes of LAB1, LAB10, and LAB11 consist of 34, 35, and 34 contigs, totalling 2.94 Mb each (GC content 37.40%). Prokka annotation predicted 2872, 2873, and 2875 protein-coding sequences (CDS) for LAB1, LAB10, and LAB11, respectively. Genomic screening revealed no vancomycin resistance genes; however, tet(M) and lsa(A) resistance determinants were identified in all three strains, located on a repUS43-type plasmid replicon. Fourteen virulence factor homologs conserved in the E. faecalis reference strain V583 were detected, including Ebp pili, gelatinase (gelE), Fsr quorum-sensing system, and capsule biosynthesis genes, but no cytolysin operon was identified. Genes associated with stress tolerance (katA, sodA), bile salt hydrolysis (cbh), siderophore transport (fepC, fhuD), and ethanolamine nitrogen metabolism (eutB/eutC) were identified in all three genomes. Pan-genome analysis with the E. faecalis reference strain revealed 551 core gene clusters and 279 gene clusters exclusive to the three aquaculture isolates. Despite their high genomic similarity, we report the three genomes as distinct isolates due to observed differences in their expressed phenotypic properties. These sequences provide a genomic resource supporting the development of multifunctional probiotic consortia for integrated aquaponic systems.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Akinyemi, Muiz Oluwatosin ; Leeds Institute of Health Sciences, University of Leeds, Leeds LS2 9LN, UK ; Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa
Coulibaly, Wahauwouele Hermann ; Université de Liège - ULiège > Département GxABT > Entomologie, Phytopathologie et Productions Innovantes (EPPI) ; Food Science and Technology Formation and Research Unit, Biotechnology and Food Microbiology Laboratory, Nangui Abrogoua University, Abidjan P.O. Box 801 02, Côte d’Ivoire
Sakia Mian, Tano Marie-Ange; Agroforestery Trainning and Research Unit, Agro-Valorization Laboratory, Jean Lorougnon Guédé University, Daloa BP-150, Côte d’Ivoire
Popescu, Paul-Alexandru ; University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., District 1, 011464 Bucharest, Romania
Ebenso, Bassey; Leeds Institute of Health Sciences, University of Leeds, Leeds LS2 9LN, UK
Razafindralambo, Hary ; Université de Liège - ULiège > Département GxABT > Microbial technologies ; ProBioLab, 5004 Namur, Belgium ; Faculty of Agricultural and Natural Resources, An Giang University, Vietnam National University, 18 Ung Van Khiem Street, Long Xuyen, An Giang 90000, Vietnam ; Ingénierie et Technologie Emergentes, Université de Vakinankaratra, Campus Vatofotsy, Antsirabe 110 BP 108, Madagascar
Language :
English
Title :
Genome Sequences of Three Enterococcus faecalis Strains (LAB1, LAB10, and LAB11) with Probiotic, Plant Growth-Promoting, and Nitrifying Properties
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