Functional characterization of fatty acid desaturases and dietary lipid sources modulation of fatty acid conversion in largemouth bass (Micropterus salmoides)

Liu, Yangyang; Caparros Megido, Rudy; Francis, Frédéric; Wang, Jie; Wang, Hao; Hu, Liang; Liang, Xiaofang; Xue, Min

doi:10.1016/j.aquaculture.2026.743641

Article (Scientific journals)

Functional characterization of fatty acid desaturases and dietary lipid sources modulation of fatty acid conversion in largemouth bass (Micropterus salmoides)

Liu, Yangyang; Caparros Megido, Rudy; Francis, Frédéric et al.

2026 • In Aquaculture, 615, p. 743641

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/347148

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10.1016/j.aquaculture.2026.743641

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Keywords :

DHA inclusion; fatty acid desaturases; Micropterus salmoides; n-3 LC-PUFA biosynthesis; oil sources; Aquatic Science

Abstract :

[en] To evaluate n-3 long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis capacity and the selected dietary lipid sources effects in largemouth bass (Micropterus salmoides), we combined functional characterization of fatty acid desaturase genes with an 8-week feeding trial. Yeast expression identified three candidate desaturase genes: fads2 (fatty acid desaturase 2) catalyzing C18:3n-3 → C18:4n-3, and delta4 fads (acyl-CoA Delta4 desaturase-like) exhibiting dual Δ5/Δ4 activity (C20:4n-3 → C20:5n-3; C22:5n-3 → C22:6n-3), and fads6 (fatty acid desaturase 6) showed no detectable desaturase activity. In a 3 × 2 factorial design, 450 juvenile largemouth bass (17.99 ± 0.02g, 25 fish per tank, triplicate) received diets containing—linseed oil (LO, 54.24% ALA), cottonseed oil (CO, 0.14% ALA) or black soldier fly oil (BSFO, 2.28% ALA), with or without DHA inclusion (3% Schizochytrium sp. meal). Two-way ANOVA revealed that LO and BSFO improved growth (FBW, WG, SGR) over CO, and LO raised tissue n-3 PUFAs levels aboved CO and BSFO (P < 0.05). CO and BSFO upregulated liver delta4 fads relative to LO, and CO further increased elovl4a and elovl5 under DHA inclusion (P < 0.05), whereas desaturase proteins remained unchanged among oils (P > 0.05). CO lowered liver TG levels and upregulated monoacylglycerol lipase (mgl) against LO and BSFO, and elevated acetyl-CoA carboxylase 1 (acc1) and fatty acid synthase (fasn) expression under DHA-inclusion (P < 0.05). Dietary DHA improved growth and tissue DHA levels, and reduced liver crude lipid and TG levels (P < 0.05), while downregulating fads2, delta4 fads, fads6 and Δ5/Δ4 Fads protein (P < 0.05). Overall, the results are consistent with largemouth bass possessing the enzymatic capacity to convert dietary ALA into n-3 LC-PUFA. Adequate dietary ALA supported growth and DHA biosynthesis, and severe ALA deficiency upregulated part n-3 LC-PUFA biosynthesis-related genes but still resulted in reduced tissue DHA. Dietary DHA inclusion alleviated growth constraints under ALA-deficient conditions.

Disciplines :

Agriculture & agronomy
Aquatic sciences & oceanology

Author, co-author :

Liu, Yangyang ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI) ; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China

Caparros Megido, Rudy ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI)

Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI)

Wang, Jie; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China

Wang, Hao; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China

Hu, Liang; Guangzhou Konfu Biotecnology Co., Ltd, Guangzhou, China

Liang, Xiaofang; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China

Xue, Min; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China

Language :

English

Title :

Functional characterization of fatty acid desaturases and dietary lipid sources modulation of fatty acid conversion in largemouth bass (Micropterus salmoides)

Publication date :

30 March 2026

Journal title :

Aquaculture

ISSN :

0044-8486

eISSN :

1873-5622

Publisher :

Elsevier B.V.

Volume :

615

Pages :

743641

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0044848626000244?httpAccept=text/xml

Funders :

CAAS - Chinese Academy of Agricultural Sciences
CSC - China Scholarship Council
NSCF - National Natural Science Foundation of China

Funding text :

This study was financially supported by the Science and Technology Program of Xinjiang Uyghur Autonomous Region ( 2024A02001 ); the National Natural Science Foundation of China ( 32273141 ; 32172981 ); and the Agricultural Science and Technology Innovation Program of CAAS , China ( CAAS-ASTIP-2023-IFR-07 ). We also gratefully acknowledge the support of the CAAS\u2013Gembloux Agro-Bio Tech Joint Training Program and the China Scholarship Council.

Available on ORBi :

since 10 July 2026

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