[en] A two-factor feeding trial evaluated the effects of soybean oil (SO) and cottonseed oil (CO), at 50 % or 100 % dietary lipid inclusion on growth, lipid metabolism, and fillet quality in largemouth bass (Micropterus salmoides). A total of 120 fish (51.66 ± 0.01 g) were assigned to four groups: SO50, CO50, SO100, CO100. All diets contained 30.2 % fishmeal, with approximately 0.8 % n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). SO diets had higher α-linolenic acid (ALA) level than CO. Growth performance was significantly affected by oil sources, with CO, particularly CO100, causing reduced final body weight (FBW), weight gain (WG) and specific growth rate (SGR), and higher feed conversation ratio (FCR). CO supplementation significantly reduced plasma triglycerides (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) and hepatic TG, corresponding with elevated adipose triglyceride lipase (atgl) expression and phosphorylated AMP-activated protein kinase (p-AMPK) levels. Fillet quality parameters also changed, with decreased water holding capacity (WHC), increased pH at 100 % vegetable oil inclusion, and improved textural properties (cohesiveness, gumminess, and chewiness) in CO diets. Although dietary ALA and desaturase gene expressions were higher in the SO groups, the n-3 LC-PUFA content in muscle and liver, as well as hepatic fatty acid desaturase (FADS) activities, did not differ significantly from those in the CO groups. In conclusion, the lack of ALA in CO, combined with limited n-3 LC-PUFA conversion capacity and the elevated lipid catabolism, particularly in the CO100 group, contributed to the inferior growth performance in largemouth bass.
Liu, Yangyang ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI) ; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Xue, Min; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI)
Caparros Megido, Rudy ; Université de Liège - ULiège > TERRA Research Centre > Entomologie, Phytopathologie et Productions Innovantes (EPPI)
Wang, Jie; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Wang, Hao; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Liang, Xiaofang ; National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Growth, fatty acid composition, and fillet quality of largemouth bass (Micropterus salmoides) fed soybean oil and cottonseed oil diets
NSCF - National Natural Science Foundation of China
Funding text :
This research was supported by Xinjiang Science and Technology Major Project ( 2024A02001\u20133 ); National Natural Science Foundation of China ( 32273141, 32172981 ); The Agricultural Science and Technology Innovation Program (ASTIP) ( CAAS-ZDRW202303 ); Beijing Innovation Consortium of Agriculture Research System ( BAIC07\u20132025\u201308 ); Beijing Natural Science Foundation ( 6232038 ); Xinjiang key Research and Development Program ( 2022B02039\u20133 ).
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