[en] Dendritic cells (DCs) are critical inducers of anti-tumor immunity. To achieve a comprehensive mapping of mouse and human DC subsets and states in a cancer context, here we generate pan-cancer mouse and human tumor-associated DC (TADC) scRNA-seq atlases, encompassing 14 mouse tumor models and 10 human cancer types, within which we identify several lineage-defined DC subsets along with maturation/functional states. We show that TADCs acquire an inflammatory profile with tumor progression and that tumor-mediated reprogramming occurs within the DCs from lymph nodes of tumor-bearing mice. Importantly, we demonstrate that TADCs are broadly conserved between mice and humans, although species-specific differences may exist in some subsets and states. Moreover, we present a comprehensive assessment of how different human TADC clusters associate with patient survival outcomes. Overall, we provide an in-depth characterization of the TADC compartment in mouse and human cancers, which can improve our understanding of the tumor microenvironment and contribute to the development of new anti-cancer therapies.
Disciplines :
Oncology
Author, co-author :
Caro, Aarushi A; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium ; Lab of Tumor Immunology and Immunotherapy, Department of Oncology, Leuven Cancer Institute, KU Leuven, Leuven, Belgium
Kancheva, Daliya ; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Hadadi, Eva; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Boeckx, Bram ; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium ; Laboratory for Translational Genetics, VIB Center for Cancer Biology, Leuven, Belgium
De Nolf, Clint ; Laboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium ; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium ; Laboratory for Barriers in Inflammation, VIB Center for Inflammation Research, Ghent, Belgium ; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Bardet, Pauline M R ; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Verstaen, Kevin ; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium ; VIB Single Cell Core, VIB, Ghent-Leuven, Belgium
Li, Luqing; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology in Lausanne (EPFL), Lausanne, Switzerland ; Agora Cancer Research Center, Lausanne, Switzerland
Elkrim, Yvon; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Vandamme, Niels; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium ; VIB Single Cell Core, VIB, Ghent-Leuven, Belgium
Deschoemaeker, Sofie ; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Blomme, Arnaud ; Université de Liège - ULiège > Département de pharmacie
Close, Pierre ; Université de Liège - ULiège > Département de pharmacie ; WELBIO Department, WEL Research Institute, Wavre, Belgium
Janssens, Sophie ; Laboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium ; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium
De Palma, Michele ; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology in Lausanne (EPFL), Lausanne, Switzerland ; Agora Cancer Research Center, Lausanne, Switzerland
Lambrechts, Diether ; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium ; Laboratory for Translational Genetics, VIB Center for Cancer Biology, Leuven, Belgium
Coosemans, An ; Lab of Tumor Immunology and Immunotherapy, Department of Oncology, Leuven Cancer Institute, KU Leuven, Leuven, Belgium
Laoui, Damya ; Lab of Dendritic Cell Biology and Cancer Immunotherapy, VIB Center for Inflammation Research, Brussels, Belgium. dlaoui@vub.be ; Lab of Cellular and Molecular Immunology, Brussels Center for Immunology, Vrije Universiteit Brussel, Brussels, Belgium. dlaoui@vub.be
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