[en] The interactions between cytokinins and ethylene on alkaloid accumulation in a periwinkle cell line have been examined. It was found that (a) either exogenously-applied cytokinins or ethylene (the latter through ethephon degradation) greatly enhanced ajmalicine accumulation in cells subcultured in a 2,4-dichlorophenoxyacetic acid-free medium; (b) the enhancing effect of cytokinin was not mediated by enhancement of endogenous ethylene production contrary to what is found in some plant models, (c) the responses to exogenous cytokinin and ethylene were additive and showed a different pattern of expression. It may be concluded that cytokinin and ethylene can up-regulate the alkaloid production in a periwinkle cells through independent pathways when added exogenously to the cultures. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
D. Gröger, Alkaloids derived from tryptophan, in: K. Mothes, H.R. Schütte, M. Luckner (Eds.), Biochemistry of alkaloids, VCH, Weinheim, 1985, pp. 273-307.
Moreno P.R.H., Van Der Heijden R., Verpoorte R. Cell and tissue culture of Catharanthus roseus: a literature survey. Plant Cell Tissue Organ Cult. 42:1995;1-25.
M.H. Zenk, H. El-Shagi, H. Arens, J. Stöckigt, E.W. Weiler, B. Deus, Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus, in: W. Barz, E. Reinhard, M.H. Zenk (Eds.), Plant Tissue Culture and its Biotechnological Application, Berlin Springer Verlag, 1977, pp. 27-44.
Smith J.L., Smart N.J., Kurtz W.G.W., Misawa M. Stimulation of indole alkaloid production in cell-suspension cultures of Catharanthus roseus by abscisic acid. Planta Med. 53:1987;470-474.
Décendit A., Liu D., Ouelhazi L., Doireau P., Mérillon J.M., Rideau M. Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures: the factors affecting the cytokinin response. Plant Cell Rep. 11:1992;400-403.
Ouelhazi L., Filali M., Décendit A., Chénieux J.C., Rideau M. Differential protein accumulation in zeatin-and 2,4-D-treated cells of Catharanthus roseus: correlation with indole alkaloid biosynthesis. Plant Physiol. Biochem. 31:1993;421-431.
P.J. Davies, The plant hormone concept: concentration, sensitivity and transport, in: P.J. Davies (Ed.), Plant Hormones. Physiology, Biochemistry and Molecular Biology, 2nd edition, Kluwer, Dordrecht, 1995, pp. 13-38.
Woodson W.R., Brandt A.S. Role of the gynoecium in cytokinin-induced carnation petal senescence. J. Am. Soc. Hortic. Sci. 116:1991;676-679.
F.B. Abeles, P.W. Morgan, M.E. Saltveit Jr., Ethylene in Plant Biology, 2nd edition, Academic Press, New York, 1992.
Bertell G., Eliasson L. Cytokinin effects on root growth and possible interactions with ethylene and indole-3-acetic acid. Physiol. Plant. 84:1992;255-261.
Cary A.J., Liu W., Howell S.H. Cytokinin action is coupled to ethylene in its effects on the inhibition of root and hypocotyl elongation in Arabidopsis thaliana seedlings. Plant Physiol. 107:1995;1075-1082.
Golan A., Tepper M., Soudry E., Horwitz B.A., Gepstein S. Cytokinin, acting through ethylene, restores gravitropism to Arabidopsis seedlings grown under red light. Plant Physiol. 112:1996;901-904.
Uegaki R., Fujimore T., Kanebo H., Kubo S., Kato K. Phytuberin and phytuberol, sesquiterpenes from Nicotiana tabacum treated with ethrel. Phytochemistry. 19:1980;1543-1544.
Milat M.L., Ricci P., Bonnet P., Blein J.P. Capsidiol and ethylene production by tobacco cells in response to cryptogen, an elicitor from Phytophthora cryptogein. Phytochemistry. 30:1991;2171-2173.
Gamborg O.L., Miller R.A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:1968;151-158.
Mérillon J.M., Ouelhazi L., Doireau P., Chénieux J.C., Rideau M. Metabolic changes and alkaloid production in habituated and non-habituated cells of Catharanthus roseus grown in hormone-free medium:. comparing hormone-deprived non-habituated cells with habituated cells. J. Plant Physiol. 134:1989;54-60.
Kevers C., Gaspar T. Vitrification of carnation in vitro: changes in ethylene production, ACC level and capacity to convert ACC to ethylene. Plant Cell Tissue Organ Cult. 4:1985;215-223.
Gamborg O.L., La Rue T.A.G. Ethylene production by plant cell cultures. The effect of auxins, abscissic acid and kinetin on ethylene production in suspension cultures of Rose and Ruta cells. Plant Physiol. 48:1971;399-401.
T.A. McKeon, J.C. Fernandez-Maculet, S.F. Yang, Biosynthesis and metabolism of ethylene, in: P.J. Davies (Ed.), Plant Hormones. Physiology, Biochemistry and Molecular Biology, 2nd edition, Kluwer, Dordrecht, 1995, pp. 118-139.
Kwalen H. Ethylene synthesis and growth in embryogenic tissues of Norway spruce: effects of oxygen, 1-aminocyclopropane-1-carboxylic acid, benzyladenine and 2,4-dichlorophenoxyacetic acid. Physiol. Plantarum. 92:1994;109-117.
Songstad D.D., Gilles K.L., Park J., Novakovski D., Epp D., Friesen L., Roewer I. Effect of ethylene on sanguinarine production from Papaver somniferum cell cultures. Plant Cell Rep. 8:1989;463-466.
Reinhardt D., Wiemken A., Boller T. Induction of ethylene biosynthesis in compatible and incompatible interactions of soybean roots with Phytophthora megasperma f. sp. glycinea and its relation to phytoalexin accumulation. J. Plant Physiol. 138:1991;394-399.
Guo Z.J., Ohta Y. Effect of ethylene biosynthesis on the accumulation of 6-methoxymellein induced by elicitors in carrot cells. J. Plant Physiol. 144:1994;700-704.
Paradies I., Konze J.R., Elstner E.F. Ethylene: indicator but not inducer of phytoalexin synthesis in soybean. Plant Physiol. 66:1980;1106-1109.
Piatti T., Boller T., Brodelius P.E. Induction of ethylene biosynthesis is correlated with but not required for induction of alkaloid accumulation in elicitor-treated Eschscholtzia cells. Phytochemistry. 30:1991;2151-2154.
Cho G.H., Kim D.I., Pedersen H., Chin C.K. Ethephon enhancement of secondary metabolite synthesis in plant cell cultures. Biotech. Prog. 4:1988;184-188.
Kim D.I, Pedersen H. Cultivation of Thalictrum rugosum cell suspension in an improved airlift bioreactor: stimulatory effect of carbon dioxide and ethylene on alkaloid production. Biotechnol. Prog. 38:1991;331-339.
Deikman J., Hammer P. E. Induction of anthocyanin accumulation by cytokinins in Arabidopsis thaliana. Plant Physiol. 108:1995;47-57.
Berglund T., Ohlsson A.B. Effects of ethylene and aminoethoxyvinylglycine on cardenolide accumulation in tissue cultures of Digitalis lanata. J. Plant Physiol. 140:1992;395-398.
Sauerbrey E., Grossman K., Jung J. Is ethylene involved in the regulation of growth of sunflower cell suspension cultures? J. Plant Physiol. 127:1987;471-479.
Garnier F., Carpin S., Label P., Crèche J., Rideau M., Hamdi S. Effect of cytokinin on alkaloid accumulation in periwinkle callus cultures transformed with a light-inducible ipt gene. Plant Sci. 120:1996;47-55.
