Convenient Genotyping of Six Myostatin Mutations Causing Double-Muscling in Cattle Using a Multiplex Oligonucleotide Ligation Assay

Karim, Latifa; Coppieters, Wouter; Grobet, Luc; Valentini, A.; Georges, Michel

doi:10.1046/j.1365-2052.2000.00684.x

Article (Scientific journals)

Convenient Genotyping of Six Myostatin Mutations Causing Double-Muscling in Cattle Using a Multiplex Oligonucleotide Ligation Assay

Karim, Latifa; Coppieters, Wouter; Grobet, Luc et al.

2000 • In Animal Genetics, 31 (6), p. 396-9

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/34420

DOI
10.1046/j.1365-2052.2000.00684.x

PubMed
11167526

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Keywords :

bovine myostatin gene; double-muscling phenotype; multiplex oligonucleotide ligation assay

Abstract :

[en] We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Karim, Latifa ; Université de Liège - ULiège > Département de productions animales > Génomique animale

Coppieters, Wouter ; Université de Liège - ULiège > Département de productions animales > Département de productions animales

Grobet, Luc ; Université de Liège - ULiège > Département de morphologie et pathologie > Embryologie

Valentini, A.

Georges, Michel ; Université de Liège - ULiège > Département de productions animales > Génomique animale

Language :

English

Title :

Convenient Genotyping of Six Myostatin Mutations Causing Double-Muscling in Cattle Using a Multiplex Oligonucleotide Ligation Assay

Publication date :

2000

Journal title :

Animal Genetics

ISSN :

0268-9146

eISSN :

1365-2052

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

Issue :

Pages :

396-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 04 January 2010

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Bibliography

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