[en] The bacterial community of the forestomach plays important roles in the digestive processes of ruminants and pseudo-ruminants. To investigate the rumen micro-biota in the pseudo-rumen of camels (Camelus dromedarius) raised in a natural environment in Algeria, bacterial diversity was determined from 25 dromedaries using 16S rRNA gene amplicon sequencing. A total of 25 bacterial phyla were identified across all the samples, including Firmicutes (close to 85℅), Bacteroidetes (about 12%) and to a lesser extent Proteobacteria (< 1%), with these three phyla together accounting for more than 97% of all sequences. Our results are consistent with previous observations of bacterial communities diversity and abundancy in the rumen or pseudo-rumen of other ruminant species (either domestic or wild), although the abundance of individual bacterial phyla showed remarkably high disparities. Links between the richness and type of diet and the composition of the rumen microbiome are discussed.
Research Center/Unit :
FARAH. Productions animales durables - ULiège
Disciplines :
Animal production & animal husbandry
Author, co-author :
Sahraoui, Naima; Saad Dahlab, Blida 1 University, Veterinary Institute of Science, Blida, Algeria
Boukert, Razika; Saad Dahlab, Blida 1 University, Veterinary Institute of Science, Blida, Algeria
Fertoul, Ayoub; Saad Dahlab, Blida 1 University, Veterinary Institute of Science, Blida, Algeria
Taminiau, Bernard ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Hornick, Jean-Luc ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Language :
English
Title :
Molecular analysis of the bacterial microbiome in the rumen of Algerian dromedary
Publication date :
13 March 2023
Journal title :
Revue d'Élevage et de Médecine Vétérinaire des Pays Tropicaux
Abbas A.M, Engelhardt W.V., Lechner Dol M., Luciano L. Mousa H.M., Reale E.,1995. Particularities in forestomach anatomy, physiology and bio-chemistry of camelids compared to ruminants. In: Tisserand J.L. (ed.). Elevage et alimentation du dromadaire. Zaragoza: CIHEAM (Options Méditer-ranéennes: Série B. Etudes et Recherches; n 13), p. 19-32
Berman H.L., McLaren M.R., Callahan B.J., 2020. Understanding and interpret-ing community sequencing measurements of the vaginal microbiome. BJOG: Int. J. Obstet. Gynaecol., 127 (2): 139-146, doi: 10.1111/1471-0528.15978
Betancur-Murillo C.L., Aguilar-Marín S.B., Jovel J., 2023: A Key Player in Ruminal Metabolism. Microorganisms, 11 (1): 1, doi: 10.3390/microorganisms11010001
Bhatt V.D., Dande S.S., Patil N.V, Joshi C.G., 2013. Molecular analysis of the bacterial microbiome in the forestomach fluid from the dromedary camel (Camelus dromedarius). Mol. Biol. Rep., 40: 3363 – 3371, doi: 10.1007/s11033-012-2411-4
Chen T., Long W., Zhang C., Liu S., Zhao L., Hamaker B.R., 2017. Fiber-Utilizing Capacity Varies in Prevotella – versus Bacteroides Dominated Gut Microbiota. Sci. Rep., 7 (1): 2594, doi: 10.1038/s41598-017-02995-4
Carberry C.A., Kenny D.A., Han S., McCabe M.S., Waters S.M., 2012. Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle. Appl. Environ. Microbiol., 78 (14): 4949– 4958, doi: 10.1128/AEM.07759-11
Deusch S., Camarinha-Silva A., Conrad J., Beifuss U., Rodehutscord M., Seif-ert J.A., 2017. Structural and Functional Elucidation of the Rumen Microbi-ome Influenced by Various Diets and Microenvironments. Front. Microbiol. 24 (8):1605, doi: 10.3389/fmicb.2017.01605
Du C., Nan X., Wang K., Zhao Y., Xiong B., 2019. Evaluation of the digestibility of steam-exploded wheat straw by ruminal fermentation, sugar yield and microbial structure in vitro. RSC Adv., 9: 41775–41782, doi: 10.1039/c9ra08167d
Flint H.J., Bayer E.A., Rincon M.T., Lamed R., White B.A., 2008. Polysaccharide utilization by gut bacteria: potential for new insights from genomics analysis. Nat. Rev. Microbiol., 6: 121–31. 65, doi: 10.1038/nrmicro1817
Gharechahi J., Zahiri H.S., Noghabi K.A., Salekdeh G.H., 2015. In-depth diversity analysis of the bacterial community resident in the camel rumen. Syst. Appl. Microbiol., 38: 67–76, doi: 10.1016/j.syapm.09.004
Gharechahi J., Salekdeh G.H.A., 2018. Metagenomic analysis of the camel rumen’s microbiome identifies the major microbes responsible for lignocellu-lose degradation and fermentation. Biotechnol. Biofuels, 11: 216, doi: 10.1186/s13068-018-1214-9
Guan Y., Zhang H., Gao X., Shang S., Wu X., Chen J., Zhang W., et al., 2016. Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene. AMB Expr 6: 98, doi: 10.1186/s13568-016-0254-4
Guan Y., Yang H., Han S., Feng L., Wang T., Ge J., 2017. Comparison of the gut microbiota composition between wild and captive sika deer (Cervus Nippon hortulorum) from feces by high-throughput sequencing. AMB Expr., 7: 212, doi: 10.1186/s13568-017-0517-8
He J., Hai L., Orgoldol K., Yi Li., Ming L., Guo F., Li G., et al., 2019. High-Throughput Sequencing Reveals the Gut Microbiome of the Bactrian Camel in Different Ages. Curr. Microbiol,.76: 810–817, doi: 10.1007/s00284-019-01689-6
Hinsu A.T., Tulsani N.J., Panchal K.J., Pandit R.J., Jyostsana B., Dafale N.A., Patil N.V., 2021. Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughage’s. Sci. rep., 11 (1): 1–14, doi: 10.1038/s41598-021-88943-9
Huws S.A., Edwards J.E., Kim E.J., Scollan N.D., 2007. Specificity and sensitivity of eubacterial primers utilized for molecular profiling of bacteria within com-plex microbial ecosystems. J. Microbiol. Meth., 70: 565–569, doi: 10.1016/j. mimet.2007.06.013
Iqbal A., Kha B.B., 2001. Feeding behaviour of camel. Review. Pak. J.Agric. Sci, 38: 58–63
Ishaq S.L., Kim C.J., Reis D., Wright A.D.G., 2015. Fibrolytic Bacteria Isolated from the Rumen of North American Moose (Alcesalces) and Their Use as a Probiotic in Neonatal Lambs. PLoS ONE, 10 (12): e0144804, doi: 10.1371/journal.pone.0144804
Jose V.L., Appoothy T., More R.P., Arun A.S., 2017. Metagenomic insights into the rumen microbial fibrolytic enzymes in Indian crossbred cattle fed finger millet straw. AMB Expr. 7: 13, doi: 10.1186/s13568-016-0310-0
Khana N.D., Zaied D.A.A., 1991. Work performance of the camel as drought and riding animal: a review. In: Wardeh M. F., Wilson R. T. (Eds.). Proceedings of International Conference on Camel Production and Improvement. Arab Centre for Studies of Arid and Dry Lands, Damascus, Syria, p. 191–201
Mc Laughlin R.W., Chen M., Zheng J., Zhao Q., Wang D., 2012. Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless por-poise, Neophocaena phocaenoides asiaeorientalis. Mol. Biol. Rep., 39 (5): 5669–5676, doi: 10.1007/s11033-011-1375-0
Madigan M., Martinko J., 2005. Brock biology of microorganisms, 11th edn. Prentice Hall, Upper Saddle River, NJ, USA, 149–152
Martens Eric C., Herbert C.C., Jerffrey I.G., 2008. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial sym-biont. Cell host & microbe, 4 (5): 447–457, doi: 10.1016/j.chom.2008.09.007
Ming L., Yi L., Sirguleng S., Hasi J., He J., Hai L., Wang Z., et al., 2017. Compar-ative analysis of fecal microbial communities in cattle and Bactrian camels. PLoSOne, 12 (3): e0173062, doi: 10.1371/journal.pone.0173062
Nguyen Cong O., Bernard T., Pham Kim D., Do Duc L., Nassim M., Nguyen Thi H., Thi H.N., et al., 2019. Growth performance, carcass quality character-istics and colonic microbiota profiles in finishing pigs fed diets with different inclusion levels of rice distillers’ by‐product. Anim. Sci. J., 90 (8): 948–960, doi: 10.1111/asj.13229
Pitta D., Kumar S., Vecchiarelli B., Shirley D.J., Bittinger K., Baker L.D., Fergu-son J.D., et al., 2014a. Temporal dynamics in the ruminal microbiome of dairy cows during the transition period. J. Anim. Sci., 92: 4014–4022, doi: 10.2527/jas.2014-7621
Pitta D.W., Parmar N., Patel A.K., Indugu N., Kumar S., Prajapathi K.B., Anand B.P., et al., 2014b. Bacterial diversity dynamics associated with different diets and different primer pairs in the rumen of Kankrej cattle. PloS one, 9: e111710, doi: 10.1371/journal.pone.0111710
Qin W., Huang Y.G., Wang Lei., Lin G., Yang J., Song P., Gao H., et al., 2020. Gut Microbiota enabled Goitered Gazelle (Gazellasubgutturosa) to Adapt to Seasonal Changes. Pakistan J. Zool., 52 (5): 1637–1646, doi: 10.17582/journal. pjz/20181031121013
Rabee A.E., Kewan K.Z., Sabra E.A., El Shaer H.M., Lamara M., 2021. Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts. Peer J., 9: e12447, doi: 10.7717/peerj.12447
Robinson T.F., Sponheimer M., Roeder B.L., Passey B., Cerling T.E., Dear-ing M.D., Ehleringer J.R., 2006. Digestibility and nitrogen retention in llamas and goats fed alfalfa, C3 grass, and C4 grasshays. Small Rumin. Res. 64: 162– 168, doi: 10.1016/j.smallrumres.2005.04.018
Samsudin A.A., Wright A.D.G., Al Jassim R., 2012. Cellulolytic bacteria in the foregut of the dromedary camel (Camelus dromedarius). Appl. Environ. Micro-biol., 78 (24): 8836–8839, doi: 10.1128/AEM.02420-12
Shang S., Liu L., Ren J., Zhang H., Zhao W., Xu J., Man Q., et al.,2019. Gutmicro-biota of the scimitar-horned oryx, Oryx dammah. Folia Zool., 68 (4): 269–273, doi: 1,025,225/fozo.019.2019
Sibtain A., Baloch Yaqoob M., Hashmi N., Shakeel A., Zaman M.A., Tariq M., 2010. Economic importance of camel: Unique alternative under crisis. Pak. Vet. J., 30 (4): 191-197
Šul’ák M., Sikorová L., Jankuvová J., Javorský P., Pristaš P., 2012 Variability of Actinobacteria, a minor component of rumen microflora. Folia Microbiol. 57: 351–353, doi: 10.1007/s12223-012-0140-7
Von Engelhardt W., Dycke C., Lechner-Doll M., 2007. Absorption of short-chain fatty acids, sodium and water from the forestomach of camels. J. Comp. Physiol. B., 177 (6): 631–640, doi: 10.1007/s00360-007-0161-8
Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., et al., 2017. Evo-lution of symbiotic bacteria in the distal human intestine. PLoS Biol., 5 (7): e156.2007, doi: 10.1371/journal.pbio.0050156
Zhang L., ChungJ., Jiang Q., Sun R., Zhang J., Zhong Y., Re N., 2017. Characteris-tics of rumen microorganisms involved in anaerobic degradation of cellulose at various pH values. RSC Adv. 7: 40303–40310, doi: 10.1039/C7RA06588D
Zhu N., Yang J., Ji L., Liu J., Yang Yand Yuan H., 2016. Metagenomic and metaproteomic analyses of a corn stover-adapted microbial consor-tium EMSD5 reveal its taxonomic and enzymatic basis for degrading lignocel-lulose. Biotechnol. Biofuels., 9: 243, doi: 10.1186/s13068-016-0658-z