Keywords :
Peptides; Proteins; 1HG84L3525 (Formaldehyde); Animals; Breast/chemistry/pathology; Breast Neoplasms/chemistry/pathology; Female; Formaldehyde/chemistry; Humans; Laser Capture Microdissection/methods; Paraffin Embedding/methods; Peptides/analysis; Proteins/analysis; Proteomics/methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods; Tissue Fixation/methods; Formalin-fixed and paraffin-embedded tissues; Histopathology; Intratumor heterogeneity; Laser microdissection; MALDI imaging; Microproteomics; Protein identification
Abstract :
[en] Matrix-assisted laser desorption ionization (MALDI) imaging is widely used for in situ proteomic mapping and finds multiple applications in pathology. However, low fragmentation yields in MALDI avoid an optimal identification of peptides from tissues. On the other hand, LMD-based microproteomic analyses allow for the identification of hundreds to thousands of proteins from small tissue regions. Herein, we present the combination of MALDI imaging and LMD-based microproteomic approaches for parallel identification. We illustrate the workflow with an application to intratumor heterogeneity studies.
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