Article (Scientific journals)
Identification of taste receptors and proteomic characterization of the antenna and legs of Tribolium brevicornis, a stored food product pest
Alabi, T; Marion-Poll, F; Danho, M et al.
2014In Insect Molecular Biology, 23 (1), p. 1 - 12
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Keywords :
chemosensory; electrophysiology; proteomic; sensilla; Tribolium; Animals; Arthropod Antennae; Chemoreceptor Cells; Insect Proteins; Microscopy, Electron, Scanning; Proteomics; Sensilla; Taste; Tribolium brevicornis; insect protein; animal; antenna (organ); article; chemoreceptor; genetics; metabolism; methodology; proteomics; scanning electron microscopy; sensillum; taste; ultrastructure
Abstract :
[en] Chemoreception plays an important role in mediating a diverse range of behaviours, including predation and food selection. In the present study, we combined anatomical observations, electrophysiology and proteomics to investigate sensilla that mediate chemoreception on the antenna and the legs of Tribolium. Scanning electron microscopy was used to differentiate the coxal and trochanteral segments of the pro-, meso- and metathoracic legs by the presence of sensilla trichoidea and chaetica, while the antennae were covered with five types of sensilla (chaetica, basiconica, trichoidea, squamiformia and coeloconica). Antenna morphology and ultrastructure were similar in both sexes. Electrophysiological recordings allowed us to characterize a row of small sensilla basiconica on the terminal segment of the antenna as taste receptors, responding to sucrose and NaCl. Proteomics investigations of antennae and legs yielded several proteins with specific interest for those involved in chemoreception. Odorant-binding proteins were antenna-specific, while chemosensory proteins were detected in both tissues. © 2013 The Royal Entomological Society.
Disciplines :
Entomology & pest control
Author, co-author :
Alabi, T;  Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, B-5030 Gembloux, Passage des Déportés, 2, Belgium ; National Polytechnic Institute FHB, ESA of Yamoussoukro, Yamoussoukro-Ivory-Cost, Cote d'Ivoire
Marion-Poll, F;  AgroParisTech Département Sciences de la Vie et Santé, Paris, France ; INRA, UMR1272 Physiologie de l'Insecte: Signalisation et Communication, Versailles, France ; Université Pierre et Marie Curie, UMR1272 Physiologie de l'Insecte: Signalisation et Communication, Versailles, France
Danho, M;  National Polytechnic Institute FHB, ESA of Yamoussoukro, Yamoussoukro-Ivory-Cost, Cote d'Ivoire
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences)
Haubruge, Eric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Identification of taste receptors and proteomic characterization of the antenna and legs of Tribolium brevicornis, a stored food product pest
Publication date :
2014
Journal title :
Insect Molecular Biology
ISSN :
0962-1075
eISSN :
1365-2583
Publisher :
Wiley, Oxford, Gb
Volume :
23
Issue :
1
Pages :
1 - 12
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 July 2025

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