Corrigendum to "Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity" [FEBS Lett. 582 (2008) 1928-1934] (DOI:10.1016/j.febslet.2008.04.043)
[en] We report on the cDNA cloning and characterization
of a novel short-chain isoprenyl diphosphate synthase from the
aphid Myzus persicae. Of the three IPPS cDNAs we cloned,
two yielded prenyltransferase activity following expression in
Escherichia coli; these cDNAs encode identical proteins except
for the presence, in one of them, of an N-terminal mitochondrial
targeting peptide. Although the aphid enzyme was predicted to
be a farnesyl diphosphate synthase by BLASTP analysis,
rMpIPPS, when isopentenyl diphosphate and dimethylallyl
diphosphate are supplied as substrates, typically generated gera-
nyl diphosphate (C10) as its main product, along with significant
quantities of farnesyl diphosphate (C15). Analysis of an
MpIPPS homology model pointed to substitutions that could
confer GPP/FPP synthase activity to the aphid enzyme.
Disciplines :
Entomology & pest control
Author, co-author :
Vandermoten, Sophie ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
CHARLOTEAUX, Benoit ; Centre Hospitalier Universitaire de Liège - CHU > > Service de génétique
Santini, Sébastien; Gembloux Agricultural University, Center of Numerical Molecular Biophysics, B-5030 Gembloux, Passage des Déportés 2, Belgium
Sen, Stephanie E; Department of Chemistry, Indiana University-Purdue University at Indianapolis (IUPUI), Indianapolis, IN 46202, 402 North Blackford Street, United States
Béliveau, Catherine; Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 Rue du PEPS, Quebec, Quebec G1V4C7, Canada
Vandenbol, Micheline ; Université de Liège - ULiège > Département GxABT > Microbial technologies
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Brasseur, Robert ; Université de Liège - ULiège > Département GxABT > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Cusson, Michel; Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 Rue du PEPS, Quebec, Quebec G1V4C7, Canada
Haubruge, Eric; Université de Liège - ULiège
Language :
English
Title :
Corrigendum to "Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity" [FEBS Lett. 582 (2008) 1928-1934] (DOI:10.1016/j.febslet.2008.04.043)
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture Bioinformatics analyses were supported by the Ministere de la Région Wallonne
