[en] This study examined the effects of fumonisins (FBs) and aflatoxin B1 (AFB1), alone or in combination, on the productivity and health of laying hens, as well as the transfer of aflatoxins (AFs) to chicken food products. The efficacy and safety of mycotoxin detoxifiers (bentonite and fumonisin esterase) to mitigate these effects were also assessed. Laying hens (400) were divided into 20 groups and fed a control, moderate (54.6 µg/kg feed) or high (546 µg/kg feed) AFB1 or FBs (7.9 mg/kg feed) added diets, either alone or in combination, with the mycotoxin detoxifiers added in selected diets. Productivity was evaluated by feed intake, egg weight, egg production, and feed conversion ratio whereas health was assessed by organ weights, blood biochemistry, and mortality. Aflatoxins residues in plasma, liver, muscle, and eggs were determined using UHPLC-MS/MS methods. A diet with AFB1 at a concentration of 546 µg/kg feed decreased egg production and various AFB1-contaminated diets increased serum uric acid levels and weights of liver, spleen, heart, and gizzard. Interactions between AFB1 and FBs significantly impacted spleen, heart, and gizzard weights as well as AFB1 residues in eggs. Maximum AFB1 residues of 0.64 µg/kg and aflatoxin M1 (below limits of quantification) were observed in liver, plasma, and eggs of layers fed diets with AFB1. The mycotoxin detoxifiers reduced effects of AFB1 and FBs on egg production, organ weights, blood biochemistry, and AFB1 residues in tissues. This study highlights the importance of mycotoxin detoxifiers as a mitigation strategy against mycotoxins in poultry production.
Disciplines :
Food science
Author, co-author :
Ochieng, Phillis Emelda ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) ; Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium
Kemboi, David C; Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium ; Department of Animal Science, Chuka University, P.O. Box 109-60400, 00625, Chuka, Kenya
Okoth, Sheila; Department of Biology, Faculty of Science and Technology, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya
De Baere, Siegrid; Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Kang'ethe, Erastus; Consultant, 00100, Nairobi, Kenya
Doupovec, Barbara; dsm-firmenich Animal Nutrition and Health R&D Center Tulln, 3430, Tulln, Austria
Gathumbi, James; Department of Veterinary Pathology, Microbiology, and Parasitology, Faculty of Veterinary Medicine, University of Nairobi, P.O. Box 29053-00100, Nairobi, Kenya
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires
Antonissen, Gunther; Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium ; Chair Poultry Health Sciences, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium
Lindahl, Johanna F; International Livestock Research Institute (ILRI), P.O. Box 30709-00100, Nairobi, Kenya. johanna.lindahl@imbim.uu.se ; Department of Medical Biochemistry and Microbiology, Uppsala University, 751 05, Uppsala, Sweden. johanna.lindahl@imbim.uu.se ; Department of Clinical Sciences, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden. johanna.lindahl@imbim.uu.se
Croubels, Siska; Department of Pathobiology, Pharmacology and Zoological Medicine, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820, Merelbeke, Belgium. Siska.Croubels@ugent.be
Language :
English
Title :
Aflatoxins and fumonisins co-contamination effects on laying hens and use of mycotoxin detoxifiers as a mitigation strategy.
Publication date :
February 2025
Journal title :
Mycotoxin Research
ISSN :
0178-7888
eISSN :
1867-1632
Publisher :
Springer Science and Business Media Deutschland GmbH, Germany
Open access funding provided by Uppsala University. This research was conducted within the ERA-NET LEAP-Agri MycoSafe-South project and was funded by the Kenyan Ministry of Education Science and Technology (MOEST), the Belgian National Fund for Scientific Research (FNRS), the Belgian Science Policy Office (BELSPO), Research Council of Norway (RCN), South Africa\u2019s National Research Foundation (NRF), BIOMIN Holding GmbH (dsm-firmenich), and Harbro Ltd. The mycotoxin detoxifiers (Mycofix\u00AE Secure and FUMzyme\u00AE) were kindly provided by Biomin\u00AE GmbH (Getzersdorf, Austria, part of dsm-firmenich). The AFs levels in biological samples were determined using a UPLC-MS/MS instrument part of the Ghent University MSsmall Expertise Centre for advanced mass spectrometry analysis of small organic molecules and partly funded by a Research Foundation Flanders (FWO) grant FWO.HMZ.2022.0008.01.Open access funding provided by Uppsala University.
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