[en] Two different procedures, alcohol precipitation with washing (APW) and a 10 kD MWCO membrane ultrafiltration with diafiltration (UF-DF), for pectin recovering and purification from crude aqueous extracts were investigated. The results showed that the yield, purity, chemical, and physicochemical features of isolated pectins depended upon the type of procedure used. The APW technique gave a higher pectin yield, and the isolated pectin contained more neutral sugars, more proteins, and more ash but less galacturonic acids than the 10 kD membrane UF-DF one. The weight-average molar mass of the APP was lower and its emulsifying properties slightly better than those of the UFPR10. Molar mass distribution of APP or UFPR10 showed two distinct pectin fractions peaks, which were separated in high-MW and low-MW pectin fractions using a 50kD MWCO membrane. The high-MW fractions exhibited poor emulsifying and emulsion stabilizing abilities whereas the low-MW fractions displayed a much higher emulsifying ability than the initial pectin fractions. Therefore, it was inferred that the ability of beet pectin to be an effective emulsifier and/or emulsion stabilizer was greatly dependent on its weight-average molar mass. (c) 2006 Elsevier Ltd. All rights reserved.
Disciplines :
Agriculture & agronomie Sciences des denrées alimentaires
Auteur, co-auteur :
Yapo, B. M.
Wathelet, Bernard ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Paquot, Michel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Langue du document :
Anglais
Titre :
Comparison of alcohol precipitation and membrane filtration effects on sugar beet pulp pectin chemical features and surface properties
Akhtar M., Dickinson E., Mazoyer J., and Langendorff V. Emulsion stabilizing properties of depolymerized pectin. Food Hydrocolloids 16 (2002) 249-256
AOAC. Official methods of analysis. 14th ed (1984), Association of Official Analytical Chemists, Washington, DC
Dea I.C.M., and Madden J.K. Acetylated pectic polysaccharides of sugar beet. Food Hydrocolloids 1 (1986) 71-88
Dickinson E. An introduction to food colloids (1992), University Press, Oxford
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., and Smith F. Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28 (1956) 350-356
Endreß H.U., and Rentschler C. Chances and limit for the use of pectin as emulsifier-Part 1. The European Food and Drink Review Summer (1999) 49-53
Garna H., Mabon N., Nott K., Wathelet B., and Paquot M. Kinetic of the hydrolysis of pectin galacturonic acid chains and quantification by ionic chromatography. Food Chemistry 96 (2006) 477-484
Hatziantoniou D., and Howell J.A. Influence of the properties and characteristics of sugar-beet pulp extract on its fouling and rejection behaviour during membrane filtration. Desalination 148 (2002) 67-72
Jiraratananon R., and Chanachai A. A study of fouling in the ultrafiltration of passion fruit juice. Journal of Membrane Science 111 (1996) 39-48
Lau A.-J., Woo S.-O., and Koh H.-L. Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection. Journal of Chromatography A 1011 (2003) 77-87
Leroux J., Langendorff V., Schick G., Vaishnav V., and Mazoyer J. Emulsion stabilizing properties of pectin. Food Hydrocolloids 17 (2003) 455-462
Mazoyer, J., Leroux, J., & Bruneau, G. (1999). Use of depolymerized citrus fruit and apple pectins as emulsifiers and emulsion stabilizers. US. Patent No. 5,900,268.
Mesbahi G., Jamalian J., and Farahnaky A. A comparative study on functional properties of beet and citrus pectins in food systems. Food Hydrocolloids 19 (2005) 731-738
Michel F., Thibault J.-F., Mercier C., Heitz F., and Pouillaude F. Extraction and characterization of pectins from sugar beet pulp. Journal of Food Science 50 (1985) 1499-1502
Morton P.A.J., and Murray B.S. Acid beverage floc: Protein-saponin interactions and an unstable emulsion model. Colloids and Surfaces B: Biointerfaces 21 (2001) 101-106
Pagán J., Ibarz A., Llorca M., and Coll L. Quality of industrial pectin extracted from peach pomace at different pH and temperatures. Journal of the Science of Food and Agriculture 79 (1999) 1038-1042
Phatak L., Chang K.C., and Brown G. Isolation and characterization of pectin in sugar-beet pulp. Journal of Food Science 53 (1988) 830-833
Rai P., Majumdar G.C., DasGupta S., and Deb S. Modeling the performance of batch ultrafiltration of synthetic fruit juice and mosambi juice using artificial neural network. Journal of Food Engineering 71 (2005) 273-281
Randall R.C., Phillips G.O., and Williams P.A. The role of the proteinaceous component on the emulsifying properties of gum arabic. Food Hydrocolloids 2 (1988) 131-140
Ridout C.L., Price K.R., Parkin G., Dijoux M.-G., and Lavaud C. Saponins from sugar beet and the floc problem. Journal of Agricultural and Food Chemistry 42 (1994) 279-282
Rombouts F.M., and Thibault J.-F. Sugar beet pectins: Chemical structure and gelation through oxidative coupling. In: Fishman M.L., and Jen J.J. (Eds). Chemistry and function of pectins (1986), American Chemical Society, Washington, DC 49-60
Shi X.Q., Chang K.C., Schwarz J.G., and Wiesenborn D. Effect of countercurrent ethanol washing on sunflower pectin quality. Carbohydrate Polymers 27 (1995) 171-175
Yapo B.M., Robert C., Etienne I., Wathelet B., and Paquot M. Effect of extraction conditions on the yield, purity and surface properties of sugar beet pulp pectin extracts. Food Chemistry (2006) in press
Yazdanshenas M., Tabatabaeenezhad A.R., Roostaazad R., and Khoshfetrat A.B. Full scale analysis of apple juice ultrafiltration and optimization of diafiltration. Separation and Purification Technology 47 (2005) 52-57
