aqueous two-phase system; flavonoids; Hawthorn leaves; response surface methodology; ultrasound; Aqueous two-phase extraction; Aqueous two-phase systems; Aqueous-two phase systems; Flavonoid; Hawthorn leaf; Optimisations; Response surfaces methods; Response-surface methodology; Test surfaces; Total flavonoids; Physics and Astronomy (all); General Physics and Astronomy
Abstract :
[en] In this paper, using hawthorn leaves as raw materials, the effect of extracting total flavonoids from hawthorn leaves by aqueous two-phase system (ATPS) combined with ultrasonic assisted was studied. Through single factor test and response surface method Box-Behnken design (response surface method, BBD), according to the regression equation, the optimal process conditions were obtained as follows: the ratio of solid to liquid 1:37, ultrasonic time 40 min, ultrasonic power 360 w, ultrasonic temperature 65°C, and the yield of flavonoids was 2.869±0.0004%. This study shows that ultrasound combined with aqueous two-phase system (ATPS) is an effective method to extract total flavonoids from hawthorn leaves.
Disciplines :
Food science
Author, co-author :
Liang, Junsong ✱; Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian, China
Zhang, Hao ✱; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian, China
Zhu, Chuanhe; Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian, China
✱ These authors have contributed equally to this work.
Language :
English
Title :
Optimization of Ultrasonic-Assisted Aqueous Two-Phase Extraction of Flavonoids from Hawthorn Leaves Using Response Surface Methodology
Publication date :
2022
Event name :
2022 2nd International Conference on Fluid and Chemical Engineering
Yin J J. Qu J G. Zhang W J. Lu D R. Gao Y C. Ying X X. Kang T G. 2014 Tissue distribution comparison between healthy and fatty liver rats after oral administration of hawthorn leaf extract Biomedical Chromatography 28 5 637 47
Min Q Bai Y Zhang Y Yu W Zhang M Liu D Diao T Lv W 2017 Hawthorn Leaf Flavonoids Protect against Diabetes-Induced Cardiomyopathy in Rats via PKC-α Signaling Pathway Evid Based Complement Alternat Med 2071952
Wang SY Xu Q Y. Zhang W J. Liu X Ying X X. Kang T G. 2011 Study on effects of hawthorn leaves extract on fatty liver in rats China Journal of Traditional Chinese Medicine and Pharmacy 26 950 955
Wang Y J. Han C H. Leng A J. Xue H F. Chen Y H. Yin J J. Lu D R. Ying X X. 2012 Pharmacokinetics of vitexin in rats after intravenous and oral administration African Journal of Pharmacy and Pharmacology 6 2368 2373
Mehmet B Selin S 2013 Effects of geographical origin and extraction methods on total phenolic yield of olive tree (Olea europaea) leaves Journal of the Taiwan Institute of Chemical Engineers 44 8 12
Chen S Xing X H. Huang J J. Xu M S. 2011 Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: Improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulose Enzyme and Microbial Technology 48 100 105
Zhang H F. Zhang X Yang X H. Qiu N X. Wang Y Wang Z Z. 2013 Microwave assisted extraction of flavonoids from cultivated Epimedium sagittatum: Extraction yield and mechanism, antioxidant activity and chemical composition Industrial Crops and Products 50 857 865
Guo Y X. Han J Zhang D Y. Wang L H. Zhou L L. 2012 An ammonium sulfate/ethanol aqueous two-phase system combined with ultrasonication for the separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge Ultrasonics Sonochemistry 19 719 724
Huang W Xue A Niu H Jia Z Wang J W. 2009 Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro Food Chemistry 114 1147 1154
Ma X Yang G Li L 2012 Aqueous two-phase extraction of flavonoids from Artemisia argyi leaves using an ethanol-ammonium sulfate system Advanced Materials Research 527 254 2258
Ying Z Han X X. Li J R. 2011 Ultrasound-assisted extraction of polysaccharides from mulberry leaves Food Chemistry 127 1273 1279
Zhang D Y. Zu Y G. Fu Y J. Wang W Zhang L Luo M Mu F S. Yao X H. Duan M H. 2013 Aqueous two-phase extraction and enrichment of two main flavonoids from pigeon pea roots and the antioxidant activity Separation and Purification Technology 102 26 33
Xiao W H. Han L J. Shi B 2008 Microwave-assisted extraction of flavonoids from Radix Astragali Separation and Purification Technology 62 614 618
