[en] [en] BACKGROUND: Research on fecal microbiota changes during hospitalization of horses with colic is emerging.
OBJECTIVES: Describe changes of the fecal microbiota during hospitalization of horses with colic caused by inflammatory (INFL), simple (SIMPLE), and strangulated (STR) obstructions, and investigate associations with survival.
ANIMALS: Twenty-three horses with colic: 9 in INFL, 5 in STR, and 9 in SIMPLE groups. Seventeen horses survived, and 6 were euthanized.
METHODS: Prospective observational study. Fecal samples were collected on admission (D1), on days 3 (D3) and 5 (D5). Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing. Data were compared using a 2-way permutational analysis of variance (PERMANOVA). Linear discriminant analysis Effect Size (LEfSE) analysis identified significant bacterial population differences, with significance set at p < 0.05 and a linear discriminant analysis (LDA) cut-off > 3.0.
RESULTS: Alpha diversity indices remained stable during hospitalization within each colic group. However, at D5, the INFL group had significantly higher richness (p < 0.01) and diversity (Shannon, p < 0.001 and Simpson, p < 0.05) than other colic types. Beta diversity (Jaccard membership and Bray-Curtis indices) was significantly different in the INFL compared to SIMPLE and STR groups (both p < 0.001) but not between SIMPLE and STR. Beta diversity membership analysis by analysis of molecular variance (AMOVA) indicated a significant difference between survivors and non-survivors within the INFL group (p < 0.01). Increased relative abundances of Bacilliculturomica and Saccharofermentans were associated with survival.
CONCLUSIONS: Microbiota showed no significant variation over 5 days of hospitalization. Colic type influenced fecal microbiota more than hospitalization duration. Specific bacterial populations may differ between survival and non-survival groups.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Loublier, Clémence ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Costa, Marcio ; Department of Veterinary Biomedicine, University of Montreal, Quebec, Canada
Taminiau, Bernard ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Lecoq, Laureline ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Amory, Hélène ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Cesarini, Carla; Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium ; Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Language :
English
Title :
Longitudinal Changes in Fecal Microbiota During Hospitalization in Horses With Different Types of Colic.
Funding: Fonds De La Recherche Scientifique\u2014FNRS (Veterinary MD. PhD. VETE-CCD) and Fonds Sp\u00E9ciaux de Recherche (DYSBIOHORSIRS). Funding provided by Fonds De La Recherche Scientifique\u2014FNRS (Veterinary MD. PhD. VETE-CCD) and Fonds Sp\u00E9ciaux de Recherche (DYSBIOHORSIRS). The authors are grateful to the veterinarians and personnel (C. Bougeard, C. George, I. Tosi, J. Ledeck, M. Bonhomme, P. Petit, B. Klein, B. Renaud, C. Wouters, P. Lejeune, the interns and externs teams) who contributed to this study. Fonds De La Recherche Scientifique—FNRS (Veterinary MD. PhD. VETE-CCD)
and Fonds Spéciaux de Recherche (DYSBIOHORSIRS).
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