Reference : The recalcitrance to rooting of the micropropagated shoots of the rac tobacco mutant:...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/3299
The recalcitrance to rooting of the micropropagated shoots of the rac tobacco mutant: Implications of polyamines and of the polyamine metabolism
English
Faivre-Rampant, Odile [Université de Liège - ULiège > > Hormonologie végétale > >]
Kevers, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Dommes, Jacques mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Gaspar, Thomas mailto [> > Botanique, radiobotanique & serres expér. >]
2000
Plant Physiology and Biochemistry
Elsevier Science
38
6
441-448
Yes (verified by ORBi)
International
0981-9428
Amsterdam
The Netherlands
[en] rooting ; tobacco mutant ; polyamines
[en] Rooting of wild-type tobacco (Nicotiana tabacum cv. Xanthi) shoots raised in vitro was promoted by polyamines in the absence of any other growth regulator and was inhibited by two inhibitors of polyamine metabolism. The auxin insensitive and recalcitrant to rooting rac mutant shoots did not respond to the same treatments. The activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), diamine oxidase (DAO), polyamine oxidase (PAO) and transglutaminases (TGases), and the titres of free and conjugated polyamines were estimated in the whole shoots and the basal parts of the stems of both tobaccos in the course of multiplication in vitro. The rac shoots grew at a lower rate. The wild-type rooted from the 7th day without special treatment. During the second week of culture, the shoots of both tobaccos were actively growing and showed an increase in ADC, ODC, DAO, PAO and TGase activities. Afterwards all these activities declined. These changes were concomitant with an increase in the polyamine contents (free and conjugated). Biosynthesis and oxidation of polyamines apparently occurred simultaneously and seemed directly correlated. In the basal part of the mutant stems however, the accumulation of free and conjugated putrescine as well as the transient increase in biosynthetic enzyme activities were delayed compared to the wild-type. These results are discussed in relation to growth behaviour and to root formation. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
Researchers
http://hdl.handle.net/2268/3299
10.1016/S0981-9428(00)00768-3

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